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- W2538479969 abstract "A rapid immunochromatographic lateral flow test strip has been developed using a gold-conjugated polyclonal antibody (pAb) for the specific determination of Cadmium (Cd <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2+</sup> ) residue in environmental water. For this purpose, the isothiocyanate method was employed to synthesize Cd <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2+</sup> artificial antigen and New Zealand white rabbits were immunized to produce anti-Cd <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2+</sup> pAb. Antigen and goat anti-rabbit IgG were respectively drawn on NC membrane as test line and control line. Gold-labeled antibody was added on a pad and put on one end of the membrane. The detection limit of this strip was measured to be 50 ng/mL. Except for high cross-reactivity (83%) toward Hg <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2+</sup> , no significant cross-reactivity was observed for other metal chelates tested. The results demonstrate that the developed method can be potentially used as a screening tool for the determination of Cd <sup xmlns:mml=http://www.w3.org/1998/Math/MathML xmlns:xlink=http://www.w3.org/1999/xlink>2+</sup> residues in a large amount of samples on site." @default.
- W2538479969 created "2016-10-28" @default.
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- W2538479969 date "2011-12-01" @default.
- W2538479969 modified "2023-09-27" @default.
- W2538479969 title "Development of a polyclonal antibody based strip assay for detection of cadmium residue" @default.
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- W2538479969 doi "https://doi.org/10.1109/iccsnt.2011.6182138" @default.
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