Matches in SemOpenAlex for { <https://semopenalex.org/work/W2538673815> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2538673815 endingPage "392" @default.
- W2538673815 startingPage "392" @default.
- W2538673815 abstract "Introduction: The existence of circulating skeletal stem cells in the peripheral blood from different species including adult mouse and human has been found and documented. The circulating skeletal stem cells may provide a new source of stem cells that may be used for bone regeneration and tissue engineering applications. The aim of this study was to investigate the existence of circulating osteogenic stem cells in the rat peripheral blood, and to compare their osteogenic potentials with bone marrow mesenchymal stem cells (BMMSCs). Methods: Whole blood from twelve female 3-month old SD rats was harvested by cardiac puncture and bone marrows were also collected. Mononuclear cells from both bone marrow and peripheral blood (PBMNCs) were isolated by Lymphoprep density gradient centrifugation method, and plated at a density of 300000 to 400000/cm2 in flasks with a-MEM medium and 15% FCS. The colony forming efficiency (CFE) was calculated after 10–14 days culture. The osteogenic, adipogenic, and chondrogenic differentiation potential of both BMMSCs and peripheral blood mononuclear cell subset were examined and compared under different specific culture conditions. In addition, both BMMSCs and peripheral blood mononuclear cell subset were seeded into absorbable porous calcium phosphate substitute and implanted subcutaneously into SCID mice for 12 weeks, and the implants were examined histologically. Results: After 10–14 days in culture, the adherent fibroblast-like colonies were formed in the PBMNCs, with CFE ranging from 1.3 to 3.5 per 10000000 cells. Under osteogenic conditions, both BMMSCs and PBMNCs subset were positive for bone markers such as ALP, type I collagen and osteocalcin; bone nodules were formed in BMMSCs and PBMNCs subset long-term culture with positive Von Kossa and Alizarin Red S staining. Under adipogenic conditions, PBMNCs subset and BMMSCs were positive for Oil Red O and C/EBP a immunostaining. For chondrogenic differentiation studies, PBMNCs subset and BMMSCs were positive for type II collagen and they had Alcian blue positive nodules formation. After implantation with calcium phosphate substitutes in SCID mice for 12 weeks, osteoid and bony tissues were evident in the implants both loaded with PBMNCs subset and BMSCSs. Conclusions: A subset of mononuclear cells that have multi-differentiation potential similar to BMMSCs exists in the rat peripheral blood. Our present study has shown that these circulating stem cells possess osteogenic potential in vitro and in vivo. Further work is ongoing to investigate the roles of PBMNCs subset in fracture healing and their recruiting and homing mechanisms." @default.
- W2538673815 created "2016-10-28" @default.
- W2538673815 creator A5001939300 @default.
- W2538673815 creator A5044244957 @default.
- W2538673815 creator A5076038393 @default.
- W2538673815 date "2006-10-01" @default.
- W2538673815 modified "2023-09-24" @default.
- W2538673815 title "THE OSTEOGENIC POTENTIAL OF A RAT PERIPHERAL BLOOD MONONUCLEAR CELL SUBSET" @default.
- W2538673815 hasPublicationYear "2006" @default.
- W2538673815 type Work @default.
- W2538673815 sameAs 2538673815 @default.
- W2538673815 citedByCount "0" @default.
- W2538673815 crossrefType "journal-article" @default.
- W2538673815 hasAuthorship W2538673815A5001939300 @default.
- W2538673815 hasAuthorship W2538673815A5044244957 @default.
- W2538673815 hasAuthorship W2538673815A5076038393 @default.
- W2538673815 hasConcept C132946820 @default.
- W2538673815 hasConcept C137061746 @default.
- W2538673815 hasConcept C142724271 @default.
- W2538673815 hasConcept C153911025 @default.
- W2538673815 hasConcept C16685009 @default.
- W2538673815 hasConcept C185592680 @default.
- W2538673815 hasConcept C198826908 @default.
- W2538673815 hasConcept C202751555 @default.
- W2538673815 hasConcept C203014093 @default.
- W2538673815 hasConcept C2780007613 @default.
- W2538673815 hasConcept C28328180 @default.
- W2538673815 hasConcept C55493867 @default.
- W2538673815 hasConcept C71924100 @default.
- W2538673815 hasConcept C86803240 @default.
- W2538673815 hasConcept C95444343 @default.
- W2538673815 hasConceptScore W2538673815C132946820 @default.
- W2538673815 hasConceptScore W2538673815C137061746 @default.
- W2538673815 hasConceptScore W2538673815C142724271 @default.
- W2538673815 hasConceptScore W2538673815C153911025 @default.
- W2538673815 hasConceptScore W2538673815C16685009 @default.
- W2538673815 hasConceptScore W2538673815C185592680 @default.
- W2538673815 hasConceptScore W2538673815C198826908 @default.
- W2538673815 hasConceptScore W2538673815C202751555 @default.
- W2538673815 hasConceptScore W2538673815C203014093 @default.
- W2538673815 hasConceptScore W2538673815C2780007613 @default.
- W2538673815 hasConceptScore W2538673815C28328180 @default.
- W2538673815 hasConceptScore W2538673815C55493867 @default.
- W2538673815 hasConceptScore W2538673815C71924100 @default.
- W2538673815 hasConceptScore W2538673815C86803240 @default.
- W2538673815 hasConceptScore W2538673815C95444343 @default.
- W2538673815 hasLocation W25386738151 @default.
- W2538673815 hasOpenAccess W2538673815 @default.
- W2538673815 hasPrimaryLocation W25386738151 @default.
- W2538673815 hasRelatedWork W1942897874 @default.
- W2538673815 hasRelatedWork W2006994674 @default.
- W2538673815 hasRelatedWork W2085662881 @default.
- W2538673815 hasRelatedWork W2111665660 @default.
- W2538673815 hasRelatedWork W2144214443 @default.
- W2538673815 hasRelatedWork W2188324574 @default.
- W2538673815 hasRelatedWork W2353383723 @default.
- W2538673815 hasRelatedWork W2355007494 @default.
- W2538673815 hasRelatedWork W2356492331 @default.
- W2538673815 hasRelatedWork W2364877867 @default.
- W2538673815 hasRelatedWork W2365907089 @default.
- W2538673815 hasRelatedWork W2375678289 @default.
- W2538673815 hasRelatedWork W2376525067 @default.
- W2538673815 hasRelatedWork W2380569029 @default.
- W2538673815 hasRelatedWork W2385674792 @default.
- W2538673815 hasRelatedWork W2386622834 @default.
- W2538673815 hasRelatedWork W2391850769 @default.
- W2538673815 hasRelatedWork W2464459931 @default.
- W2538673815 hasRelatedWork W2974731822 @default.
- W2538673815 hasRelatedWork W3031457455 @default.
- W2538673815 isParatext "false" @default.
- W2538673815 isRetracted "false" @default.
- W2538673815 magId "2538673815" @default.
- W2538673815 workType "article" @default.