Matches in SemOpenAlex for { <https://semopenalex.org/work/W2538719953> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2538719953 abstract "Abstract In the last steps of differentiation mammalian erythroid cells undergo chromatin condensation and enucleation; the latter process does not occur in other vertebrates. Enucleation was critical for the evolution of mammals, as it permits an enhanced hemoglobin concentration – and thus oxygen- carrying capacity – in mammalian red blood cells. We previously reported that Rac GTPases and their downstream forming target mDia2 are required for mouse fetal erythroblast enucleation. We also found that the nucleus undergoes a gradual ~10- fold decrease in volume during erythropoiesis. Since histone deacetylases (HDACs) play important roles in chromatin condensation, we hypothesized that HDACs are involved in mammalian erythroblast enucleation. To test this hypothesis, we purified E13.5 mouse fetal TER119 negative erythroblasts and cultured them in fibronectin-coated plates. Cells were then treated with Trichostatin A (TSA), a pan-HDAC inhibitor, at different times during erythropoiesis. TSA completely blocked enucleation and kinetic studies showed this inhibitory effect occurred earlier than the step catalyzed by the Rac-mDia2 pathway. This indicates that chromatin condensation is required for the final extrusion of the nucleus. We further investigated the activity ofHDAC6 specifically, since HDAC6 is known to interact with mDia2. We found that mDia2is acetylated in vitro and in vivo and that HDAC6 interacts with and deacetylates mDia2. Treatment of purified TER119 negative mouse fetal erythroblasts with a specific HDAC6inhibitor partially blocked enucleation. We conclude that histone deacetylase activities are essential for mammalian erythroblasts to undergo enucleation and that HDACs act both on chromatin condensation and mDia2 deacetylation to promote enucleation." @default.
- W2538719953 created "2016-10-28" @default.
- W2538719953 creator A5027055922 @default.
- W2538719953 creator A5042912398 @default.
- W2538719953 creator A5076111394 @default.
- W2538719953 date "2008-11-16" @default.
- W2538719953 modified "2023-09-29" @default.
- W2538719953 title "Histone Deacetylases Are Essential for Chromatin Condensation and Enucleation in Mammalian Erythroblasts" @default.
- W2538719953 doi "https://doi.org/10.1182/blood.v112.11.1375.1375" @default.
- W2538719953 hasPublicationYear "2008" @default.
- W2538719953 type Work @default.
- W2538719953 sameAs 2538719953 @default.
- W2538719953 citedByCount "0" @default.
- W2538719953 crossrefType "journal-article" @default.
- W2538719953 hasAuthorship W2538719953A5027055922 @default.
- W2538719953 hasAuthorship W2538719953A5042912398 @default.
- W2538719953 hasAuthorship W2538719953A5076111394 @default.
- W2538719953 hasConcept C104317684 @default.
- W2538719953 hasConcept C109159458 @default.
- W2538719953 hasConcept C126322002 @default.
- W2538719953 hasConcept C148196450 @default.
- W2538719953 hasConcept C153911025 @default.
- W2538719953 hasConcept C185592680 @default.
- W2538719953 hasConcept C188261085 @default.
- W2538719953 hasConcept C2777865847 @default.
- W2538719953 hasConcept C2778248108 @default.
- W2538719953 hasConcept C2778305200 @default.
- W2538719953 hasConcept C2779703530 @default.
- W2538719953 hasConcept C28328180 @default.
- W2538719953 hasConcept C2909030593 @default.
- W2538719953 hasConcept C45413362 @default.
- W2538719953 hasConcept C54355233 @default.
- W2538719953 hasConcept C55493867 @default.
- W2538719953 hasConcept C64927066 @default.
- W2538719953 hasConcept C71924100 @default.
- W2538719953 hasConcept C83640560 @default.
- W2538719953 hasConcept C86803240 @default.
- W2538719953 hasConcept C95444343 @default.
- W2538719953 hasConceptScore W2538719953C104317684 @default.
- W2538719953 hasConceptScore W2538719953C109159458 @default.
- W2538719953 hasConceptScore W2538719953C126322002 @default.
- W2538719953 hasConceptScore W2538719953C148196450 @default.
- W2538719953 hasConceptScore W2538719953C153911025 @default.
- W2538719953 hasConceptScore W2538719953C185592680 @default.
- W2538719953 hasConceptScore W2538719953C188261085 @default.
- W2538719953 hasConceptScore W2538719953C2777865847 @default.
- W2538719953 hasConceptScore W2538719953C2778248108 @default.
- W2538719953 hasConceptScore W2538719953C2778305200 @default.
- W2538719953 hasConceptScore W2538719953C2779703530 @default.
- W2538719953 hasConceptScore W2538719953C28328180 @default.
- W2538719953 hasConceptScore W2538719953C2909030593 @default.
- W2538719953 hasConceptScore W2538719953C45413362 @default.
- W2538719953 hasConceptScore W2538719953C54355233 @default.
- W2538719953 hasConceptScore W2538719953C55493867 @default.
- W2538719953 hasConceptScore W2538719953C64927066 @default.
- W2538719953 hasConceptScore W2538719953C71924100 @default.
- W2538719953 hasConceptScore W2538719953C83640560 @default.
- W2538719953 hasConceptScore W2538719953C86803240 @default.
- W2538719953 hasConceptScore W2538719953C95444343 @default.
- W2538719953 hasLocation W25387199531 @default.
- W2538719953 hasOpenAccess W2538719953 @default.
- W2538719953 hasPrimaryLocation W25387199531 @default.
- W2538719953 hasRelatedWork W1972372000 @default.
- W2538719953 hasRelatedWork W1972724678 @default.
- W2538719953 hasRelatedWork W1987739511 @default.
- W2538719953 hasRelatedWork W2009906193 @default.
- W2538719953 hasRelatedWork W2019372604 @default.
- W2538719953 hasRelatedWork W2023221086 @default.
- W2538719953 hasRelatedWork W2040572666 @default.
- W2538719953 hasRelatedWork W2110938577 @default.
- W2538719953 hasRelatedWork W2132814931 @default.
- W2538719953 hasRelatedWork W2152480074 @default.
- W2538719953 hasRelatedWork W2180646329 @default.
- W2538719953 hasRelatedWork W2193979452 @default.
- W2538719953 hasRelatedWork W2402464252 @default.
- W2538719953 hasRelatedWork W2561298110 @default.
- W2538719953 hasRelatedWork W2578513127 @default.
- W2538719953 hasRelatedWork W2759934086 @default.
- W2538719953 hasRelatedWork W2765877302 @default.
- W2538719953 hasRelatedWork W3005432137 @default.
- W2538719953 hasRelatedWork W3139285620 @default.
- W2538719953 hasRelatedWork W3155403007 @default.
- W2538719953 isParatext "false" @default.
- W2538719953 isRetracted "false" @default.
- W2538719953 magId "2538719953" @default.
- W2538719953 workType "article" @default.