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• W2538989011 abstract "The field of Alzheimer’s Disease (AD) requires a protein biomarker panel for early diagnosis, differential diagnosis, to predict the rate of cognitive decline (= progression biomarker(s)), to allow a better identification of affected subjects (= patient stratification). Synapse proteins (eg, neurogranin, synuclein) have been shown to correlate better with memory decline compared to tau and ß-Amyloid (Aß). In order to fasten their integration in studies world-wide, it is important to verify the impact of pre-analytical parameters on the accuracy of neurogranin and α-synuclein measurements. Identical procedures as optimized for Aß(1-42) are of utmost importance. CSF, collected from 19 subjects, was evaluated with respect to collection and storage recipients (low binding (LoB), polypropylene (PP)), total volume of tubes (0.5 mL, 1.5 mL), number of freeze/thaw cycles (1,2), and addition of detergents (0.05% v/v Triton X-100, Tween-20) at the moment of CSF collection. Neurogranin (truncated at position 75) and α-synuclein analytes were measured with absorbance based ELISA methods using newly developed monoclonal antibodies. The data have been analyzed with linear repeated measures and mixed effects models. Concentrations of α-synuclein and neurogranin trunc P75 are less affected by recipient surface or volume of the tubes than Aß proteins. An additional freeze-thaw cycle resulted in slight, but significant reduction of the neurogranin trunc P75 concentrations, but not for α-synuclein. This is especially the case for CSF collected in PP tubes with a higher total volume. The difference in analyte concentrations in function of the collection protocol can be tapered by addition of a detergent at the time of CSF collection. This is the first extensive study in which pre-analytical variables for analysis of neurogranin trunc P75 and α-synuclein have been studied in extenso, in parallel with the classical biomarkers. Our results show that neurogranin trunc P75 levels, in contrast to α-synuclein, is also affected (although to a lesser extent than Aß(1-42)) by the number of freeze-thaw cycles. It can be concluded that a protocol for CSF collection of CSF Aß(1-42) can be integrated in procedures for analysis of neurogranin trunc P75 and α-synuclein ." @default.
• W2538989011 created "2016-10-28" @default.
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• W2538989011 date "2016-07-01" @default.
• W2538989011 modified "2023-10-16" @default.
• W2538989011 title "P4-316: Standardization of Pre-Analytical Procedures for Collection and Storage of CSF for the Measurement of Neurogranin Trunc P75 and a-Synuclein" @default.
• W2538989011 doi "https://doi.org/10.1016/j.jalz.2016.07.059" @default.
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