FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2540094577> ?p ?o ?g. }

• W2540094577 endingPage "1867" @default.
• W2540094577 startingPage "1859" @default.
• W2540094577 abstract "RNA interference via small interfering RNA (siRNA) has many potential therapeutic applications, and liposomal-based systems are useful for improving the pharmacokinetics of siRNAs, including their intracellular release and distribution. However, for the successful translation of this technology into clinical applications, it is important to understand how liposomal encapsulation changes the cellular uptake and immunostimulatory adverse effects of siRNAs. Here we evaluated the cellular uptake and innate immune activation by an immunostimulatory siRNA encapsulated within a liposome carrier in commercially available human peripheral blood mononuclear cells (PBMCs). We found considerable lot-to-lot variation in cytokine production by the PBMCs. Flow cytometric analysis in conjunction with intracellular staining of tumor necrosis factor-α (TNF-α) revealed that after treating PBMCs with the liposomal siRNA, approximately 5% of the cells produced TNF-α and more than 90% of the TNF-α-producing cells were positive for CD14 expression. We also showed that peripheral blood CD14+ monocytes in the cytokine release assay had low inter-lot variabilities in TNF-α production, suggesting that the peripheral blood CD14+ monocyte-based cytokine release assay is a specific means of alleviating the lot-to-lot variability in the cytokine release profiles of commercially available PBMCs. Our results also show that the peripheral blood CD14+ monocyte-based cytokine release assay can be used to identify the siRNA recognition receptors that mediate individual cytokine production." @default.
• W2540094577 created "2016-11-04" @default.
• W2540094577 creator A5028835943 @default.
• W2540094577 creator A5044208662 @default.
• W2540094577 creator A5053797637 @default.
• W2540094577 date "2016-01-01" @default.
• W2540094577 modified "2023-10-18" @default.
• W2540094577 title "Cell Type-Specific Responses of Peripheral Blood CD14-Positive Monocytes to Liposome-Encapsulated Immunostimulatory siRNA" @default.
• W2540094577 cites W1511669342 @default.
• W2540094577 cites W1515466910 @default.
• W2540094577 cites W1611419331 @default.
• W2540094577 cites W1669278039 @default.
• W2540094577 cites W1705666194 @default.
• W2540094577 cites W1951507118 @default.
• W2540094577 cites W1965859404 @default.
• W2540094577 cites W1966334833 @default.
• W2540094577 cites W1967142707 @default.
• W2540094577 cites W1969072247 @default.
• W2540094577 cites W1969354039 @default.
• W2540094577 cites W1972841312 @default.
• W2540094577 cites W1984574632 @default.
• W2540094577 cites W1985069370 @default.
• W2540094577 cites W1991236592 @default.
• W2540094577 cites W1992645970 @default.
• W2540094577 cites W2000787206 @default.
• W2540094577 cites W2002075586 @default.
• W2540094577 cites W2013947977 @default.
• W2540094577 cites W2020368715 @default.
• W2540094577 cites W2025980739 @default.
• W2540094577 cites W2027268325 @default.
• W2540094577 cites W2027954222 @default.
• W2540094577 cites W2029827106 @default.
• W2540094577 cites W2030621748 @default.
• W2540094577 cites W2040207921 @default.
• W2540094577 cites W2041649971 @default.
• W2540094577 cites W2053202538 @default.
• W2540094577 cites W2057479883 @default.
• W2540094577 cites W2062991743 @default.
• W2540094577 cites W2065135000 @default.
• W2540094577 cites W2071206211 @default.
• W2540094577 cites W2072301209 @default.
• W2540094577 cites W2075295096 @default.
• W2540094577 cites W2077897130 @default.
• W2540094577 cites W2085217622 @default.
• W2540094577 cites W2098777190 @default.
• W2540094577 cites W2107526714 @default.
• W2540094577 cites W2109379806 @default.
• W2540094577 cites W2114208662 @default.
• W2540094577 cites W2119293746 @default.
• W2540094577 cites W2121133384 @default.
• W2540094577 cites W2122234458 @default.
• W2540094577 cites W2124059797 @default.
• W2540094577 cites W2125491088 @default.
• W2540094577 cites W2147763501 @default.
• W2540094577 cites W2148919642 @default.
• W2540094577 cites W2151777859 @default.
• W2540094577 cites W2157717331 @default.
• W2540094577 cites W2162692691 @default.
• W2540094577 doi "https://doi.org/10.1248/bpb.b16-00450" @default.
• W2540094577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27803458" @default.
• W2540094577 hasPublicationYear "2016" @default.
• W2540094577 type Work @default.
• W2540094577 sameAs 2540094577 @default.
• W2540094577 citedByCount "1" @default.
• W2540094577 countsByYear W25400945772021 @default.
• W2540094577 crossrefType "journal-article" @default.
• W2540094577 hasAuthorship W2540094577A5028835943 @default.
• W2540094577 hasAuthorship W2540094577A5044208662 @default.
• W2540094577 hasAuthorship W2540094577A5053797637 @default.
• W2540094577 hasBestOaLocation W25400945771 @default.
• W2540094577 hasConcept C137061746 @default.
• W2540094577 hasConcept C17991360 @default.
• W2540094577 hasConcept C202751555 @default.
• W2540094577 hasConcept C203014093 @default.
• W2540094577 hasConcept C22615655 @default.
• W2540094577 hasConcept C2778513237 @default.
• W2540094577 hasConcept C2778690821 @default.
• W2540094577 hasConcept C2781184567 @default.
• W2540094577 hasConcept C54009773 @default.
• W2540094577 hasConcept C54355233 @default.
• W2540094577 hasConcept C55493867 @default.
• W2540094577 hasConcept C81885089 @default.
• W2540094577 hasConcept C86803240 @default.
• W2540094577 hasConcept C8891405 @default.
• W2540094577 hasConcept C98274493 @default.
• W2540094577 hasConceptScore W2540094577C137061746 @default.
• W2540094577 hasConceptScore W2540094577C17991360 @default.
• W2540094577 hasConceptScore W2540094577C202751555 @default.
• W2540094577 hasConceptScore W2540094577C203014093 @default.
• W2540094577 hasConceptScore W2540094577C22615655 @default.
• W2540094577 hasConceptScore W2540094577C2778513237 @default.
• W2540094577 hasConceptScore W2540094577C2778690821 @default.
• W2540094577 hasConceptScore W2540094577C2781184567 @default.
• W2540094577 hasConceptScore W2540094577C54009773 @default.
• W2540094577 hasConceptScore W2540094577C54355233 @default.
• W2540094577 hasConceptScore W2540094577C55493867 @default.
• W2540094577 hasConceptScore W2540094577C81885089 @default.
• W2540094577 hasConceptScore W2540094577C86803240 @default.

• next