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- W254132015 abstract "Low yield of a thermostable pectinesterase(TSPE) from citrus fruits has made its detailed study extremely tedious and difficult; therefore, maximizing TSPE extraction is desirable. It is assumed that TSPE is bound to the cell components via ionic linkage and covalent bonds. Therefore, in this study, variations in extraction time, pH, NaCl concentration and commercial enzyme preparations were used to increase the yield of TSPE from Valencia orange. The largest recovery of TSPE, obtained by heating extracted pectinesterase(PE) at minute, was achieved using actate buffer(pH 4.14) with 1 M NaCl and 0.2% 104(a mixture of cellulase, hemicellulase and pectinase; Genecor, Inc). The two aquous phase partitioning with 5.0% Triton X-114 could be used as a tool for separation of thermolabile pectinesterase(TLPE) and TSPE from crude PE. Also, water extraction and ammonium sulfate fractionation could be used for removing non-pectinesterase protein." @default.
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- W254132015 date "1995-01-01" @default.
- W254132015 modified "2023-09-23" @default.
- W254132015 title "A Study on the Extraction of Thermostable Pectinesterase from Valencia Orange" @default.
- W254132015 hasPublicationYear "1995" @default.
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