Matches in SemOpenAlex for { <https://semopenalex.org/work/W2544877029> ?p ?o ?g. }
- W2544877029 endingPage "1808" @default.
- W2544877029 startingPage "1808" @default.
- W2544877029 abstract "We report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800-1800 cm-1) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm-1) and for the selection of the two internal standards (DNA at 786 cm-1 and Amide I at 1662 cm-1). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts-determined by gas chromatography-from 12% to 90% (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure-from bacteria sampling, centrifugation, and sample preparation to Raman analysis-can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h." @default.
- W2544877029 created "2016-11-04" @default.
- W2544877029 creator A5001418330 @default.
- W2544877029 creator A5005181870 @default.
- W2544877029 creator A5005948607 @default.
- W2544877029 creator A5054904756 @default.
- W2544877029 creator A5061192722 @default.
- W2544877029 creator A5062513693 @default.
- W2544877029 creator A5069740803 @default.
- W2544877029 creator A5071578368 @default.
- W2544877029 creator A5076516552 @default.
- W2544877029 creator A5091184798 @default.
- W2544877029 date "2016-10-28" @default.
- W2544877029 modified "2023-10-16" @default.
- W2544877029 title "Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16" @default.
- W2544877029 cites W1539921850 @default.
- W2544877029 cites W1963638325 @default.
- W2544877029 cites W1969098225 @default.
- W2544877029 cites W1974403844 @default.
- W2544877029 cites W1979165463 @default.
- W2544877029 cites W1987157188 @default.
- W2544877029 cites W2002661354 @default.
- W2544877029 cites W2011008984 @default.
- W2544877029 cites W2019178393 @default.
- W2544877029 cites W2034180958 @default.
- W2544877029 cites W2034945050 @default.
- W2544877029 cites W2035764379 @default.
- W2544877029 cites W2044573491 @default.
- W2544877029 cites W2052340050 @default.
- W2544877029 cites W2060709376 @default.
- W2544877029 cites W2077340481 @default.
- W2544877029 cites W2092345857 @default.
- W2544877029 cites W2095129805 @default.
- W2544877029 cites W2101225670 @default.
- W2544877029 cites W2110255633 @default.
- W2544877029 cites W2118196438 @default.
- W2544877029 cites W2119181590 @default.
- W2544877029 cites W2136638355 @default.
- W2544877029 cites W2163954429 @default.
- W2544877029 cites W2480655272 @default.
- W2544877029 doi "https://doi.org/10.3390/s16111808" @default.
- W2544877029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5134467" @default.
- W2544877029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27801828" @default.
- W2544877029 hasPublicationYear "2016" @default.
- W2544877029 type Work @default.
- W2544877029 sameAs 2544877029 @default.
- W2544877029 citedByCount "23" @default.
- W2544877029 countsByYear W25448770292017 @default.
- W2544877029 countsByYear W25448770292018 @default.
- W2544877029 countsByYear W25448770292019 @default.
- W2544877029 countsByYear W25448770292020 @default.
- W2544877029 countsByYear W25448770292021 @default.
- W2544877029 countsByYear W25448770292022 @default.
- W2544877029 crossrefType "journal-article" @default.
- W2544877029 hasAuthorship W2544877029A5001418330 @default.
- W2544877029 hasAuthorship W2544877029A5005181870 @default.
- W2544877029 hasAuthorship W2544877029A5005948607 @default.
- W2544877029 hasAuthorship W2544877029A5054904756 @default.
- W2544877029 hasAuthorship W2544877029A5061192722 @default.
- W2544877029 hasAuthorship W2544877029A5062513693 @default.
- W2544877029 hasAuthorship W2544877029A5069740803 @default.
- W2544877029 hasAuthorship W2544877029A5071578368 @default.
- W2544877029 hasAuthorship W2544877029A5076516552 @default.
- W2544877029 hasAuthorship W2544877029A5091184798 @default.
- W2544877029 hasBestOaLocation W25448770291 @default.
- W2544877029 hasConcept C119394753 @default.
- W2544877029 hasConcept C120665830 @default.
- W2544877029 hasConcept C121332964 @default.
- W2544877029 hasConcept C123460561 @default.
- W2544877029 hasConcept C178790620 @default.
- W2544877029 hasConcept C185592680 @default.
- W2544877029 hasConcept C192562407 @default.
- W2544877029 hasConcept C2780637011 @default.
- W2544877029 hasConcept C40003534 @default.
- W2544877029 hasConcept C43617362 @default.
- W2544877029 hasConcept C523546767 @default.
- W2544877029 hasConcept C54355233 @default.
- W2544877029 hasConcept C73447357 @default.
- W2544877029 hasConcept C86803240 @default.
- W2544877029 hasConcept C95986675 @default.
- W2544877029 hasConceptScore W2544877029C119394753 @default.
- W2544877029 hasConceptScore W2544877029C120665830 @default.
- W2544877029 hasConceptScore W2544877029C121332964 @default.
- W2544877029 hasConceptScore W2544877029C123460561 @default.
- W2544877029 hasConceptScore W2544877029C178790620 @default.
- W2544877029 hasConceptScore W2544877029C185592680 @default.
- W2544877029 hasConceptScore W2544877029C192562407 @default.
- W2544877029 hasConceptScore W2544877029C2780637011 @default.
- W2544877029 hasConceptScore W2544877029C40003534 @default.
- W2544877029 hasConceptScore W2544877029C43617362 @default.
- W2544877029 hasConceptScore W2544877029C523546767 @default.
- W2544877029 hasConceptScore W2544877029C54355233 @default.
- W2544877029 hasConceptScore W2544877029C73447357 @default.
- W2544877029 hasConceptScore W2544877029C86803240 @default.
- W2544877029 hasConceptScore W2544877029C95986675 @default.
- W2544877029 hasFunder F4320321006 @default.
- W2544877029 hasIssue "11" @default.
- W2544877029 hasLocation W25448770291 @default.