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- W2545013763 abstract "Rapid and sensitive point-of-care diagnostics are of paramount importance for early detection of infectious diseases and timely initiation of treatment. Here, we present cellulose paper and flexible plastic chips with printed graphene-modified silver electrodes as universal point-of-care diagnostic tools for the rapid and sensitive detection of microbial pathogens or nucleic acids through utilizing electrical sensing modality and loop-mediated isothermal amplification (LAMP). We evaluated the ability of the developed paper-based assay to detect (i) viruses on cellulose-based paper microchips without implementing amplification in samples with viral loads between 106 and 108 copies per ml, and (ii) amplified HIV-1 nucleic acids in samples with viral loads between 10 fg μl−1 and 108 fg μl−1. The target HIV-1 nucleic acid was amplified using the RT-LAMP technique and detected through the electrical sensing of LAMP amplicons for a broad range of RNA concentrations between 10 fg μl−1 and 108 fg μl−1 after 40 min of amplification time. Our assay may be used for antiretroviral therapy monitoring where it meets the sensitivity requirement of the World Health Organization guidelines. Such a paper microchip assay without the amplification step may also be considered as a simple and inexpensive approach for acute HIV detection where maximum viral replication occurs." @default.
- W2545013763 created "2016-11-04" @default.
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- W2545013763 creator A5085727500 @default.
- W2545013763 date "2017-01-01" @default.
- W2545013763 modified "2023-10-15" @default.
- W2545013763 title "Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens" @default.
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- W2545013763 doi "https://doi.org/10.1039/c6nr06417e" @default.
- W2545013763 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5695240" @default.
- W2545013763 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27845796" @default.
- W2545013763 hasPublicationYear "2017" @default.
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