FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2545286687> ?p ?o ?g. }

• W2545286687 abstract "Although fusion of somatic cells with embryonic stem (ES) cells has been shown to induce reprogramming, single-cell level details of the transitory phenotypic changes that occur during fusion-based reprogramming are still lacking. Our group previously reported on the technique of one-to-one electrofusion via micro-slits in a microfluidic platform. In this study, we focused on developing a novel air-lock patterning technique for creating localized adhesion zones around the micro-slits for cell localization and real-time imaging of post fusion events with a single-cell resolution. Mouse embryonic fibroblasts (MEF) were fused individually with mouse ES cells using a polydimethylsiloxane (PDMS) fusion chip consisting of two feeder channels with a separating wall containing an array of micro-slits (slit width ∼3 μm) at a regular spacing. ES cells and MEFs were introduced separately into the channels, juxtaposed on the micro-slits by dielectrophoresis and fused one-to-one by a pulse voltage. To localize fused cells for on-chip culture and time-lapse microscopy, we implemented a two-step approach of air-lock bovine serum albumin patterning and Matrigel coating to create localized adhesion areas around the micro-slits. As a result of time-lapse imaging, we could determine that cell division occurs within 24 h after fusion, much earlier than the 2–3 days reported by earlier studies. Remarkably, Oct4-GFP (Green Fluorescent Protein) was confirmed after 25 h of fusion and thereafter stably expressed by daughter cells of fused cells. Thus, integrated into our high-yield electrofusion platform, the technique of air-lock assisted adhesion patterning enables a single-cell level tracking of fused cells to highlight cell-level dynamics during fusion-based reprogramming." @default.
• W2545286687 created "2016-11-04" @default.
• W2545286687 creator A5012327593 @default.
• W2545286687 creator A5018343564 @default.
• W2545286687 creator A5018447144 @default.
• W2545286687 creator A5035024233 @default.
• W2545286687 creator A5046320601 @default.
• W2545286687 creator A5055250823 @default.
• W2545286687 creator A5065666549 @default.
• W2545286687 date "2016-09-01" @default.
• W2545286687 modified "2023-10-12" @default.
• W2545286687 title "Adhesion patterning by a novel air-lock technique enables localization and <i>in-situ</i> real-time imaging of reprogramming events in one-to-one electrofused hybrids" @default.
• W2545286687 cites W139247133 @default.
• W2545286687 cites W1833941303 @default.
• W2545286687 cites W1835164802 @default.
• W2545286687 cites W1969674659 @default.
• W2545286687 cites W1976685409 @default.
• W2545286687 cites W1981492935 @default.
• W2545286687 cites W1992134339 @default.
• W2545286687 cites W1999274842 @default.
• W2545286687 cites W2020246476 @default.
• W2545286687 cites W2052002403 @default.
• W2545286687 cites W2059466663 @default.
• W2545286687 cites W2070160283 @default.
• W2545286687 cites W2072582405 @default.
• W2545286687 cites W2075489180 @default.
• W2545286687 cites W2085489424 @default.
• W2545286687 cites W2089426706 @default.
• W2545286687 cites W2097920749 @default.
• W2545286687 cites W2100742281 @default.
• W2545286687 cites W2109707676 @default.
• W2545286687 cites W2118624932 @default.
• W2545286687 cites W2139705669 @default.
• W2545286687 cites W2142783700 @default.
• W2545286687 cites W2152526860 @default.
• W2545286687 cites W2156126035 @default.
• W2545286687 cites W2161815644 @default.
• W2545286687 cites W2170301487 @default.
• W2545286687 cites W289241723 @default.
• W2545286687 cites W4239976151 @default.
• W2545286687 doi "https://doi.org/10.1063/1.4965422" @default.
• W2545286687 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5085977" @default.
• W2545286687 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27822330" @default.
• W2545286687 hasPublicationYear "2016" @default.
• W2545286687 type Work @default.
• W2545286687 sameAs 2545286687 @default.
• W2545286687 citedByCount "5" @default.
• W2545286687 countsByYear W25452866872017 @default.
• W2545286687 countsByYear W25452866872019 @default.
• W2545286687 countsByYear W25452866872022 @default.
• W2545286687 countsByYear W25452866872023 @default.
• W2545286687 crossrefType "journal-article" @default.
• W2545286687 hasAuthorship W2545286687A5012327593 @default.
• W2545286687 hasAuthorship W2545286687A5018343564 @default.
• W2545286687 hasAuthorship W2545286687A5018447144 @default.
• W2545286687 hasAuthorship W2545286687A5035024233 @default.
• W2545286687 hasAuthorship W2545286687A5046320601 @default.
• W2545286687 hasAuthorship W2545286687A5055250823 @default.
• W2545286687 hasAuthorship W2545286687A5065666549 @default.
• W2545286687 hasBestOaLocation W25452866871 @default.
• W2545286687 hasConcept C104317684 @default.
• W2545286687 hasConcept C12554922 @default.
• W2545286687 hasConcept C142613039 @default.
• W2545286687 hasConcept C145103041 @default.
• W2545286687 hasConcept C1491633281 @default.
• W2545286687 hasConcept C159985019 @default.
• W2545286687 hasConcept C171250308 @default.
• W2545286687 hasConcept C185592680 @default.
• W2545286687 hasConcept C191897082 @default.
• W2545286687 hasConcept C192562407 @default.
• W2545286687 hasConcept C2776609970 @default.
• W2545286687 hasConcept C2778608917 @default.
• W2545286687 hasConcept C2779849746 @default.
• W2545286687 hasConcept C55493867 @default.
• W2545286687 hasConcept C77255625 @default.
• W2545286687 hasConcept C84416704 @default.
• W2545286687 hasConcept C85789140 @default.
• W2545286687 hasConcept C86803240 @default.
• W2545286687 hasConcept C92488253 @default.
• W2545286687 hasConcept C95444343 @default.
• W2545286687 hasConceptScore W2545286687C104317684 @default.
• W2545286687 hasConceptScore W2545286687C12554922 @default.
• W2545286687 hasConceptScore W2545286687C142613039 @default.
• W2545286687 hasConceptScore W2545286687C145103041 @default.
• W2545286687 hasConceptScore W2545286687C1491633281 @default.
• W2545286687 hasConceptScore W2545286687C159985019 @default.
• W2545286687 hasConceptScore W2545286687C171250308 @default.
• W2545286687 hasConceptScore W2545286687C185592680 @default.
• W2545286687 hasConceptScore W2545286687C191897082 @default.
• W2545286687 hasConceptScore W2545286687C192562407 @default.
• W2545286687 hasConceptScore W2545286687C2776609970 @default.
• W2545286687 hasConceptScore W2545286687C2778608917 @default.
• W2545286687 hasConceptScore W2545286687C2779849746 @default.
• W2545286687 hasConceptScore W2545286687C55493867 @default.
• W2545286687 hasConceptScore W2545286687C77255625 @default.
• W2545286687 hasConceptScore W2545286687C84416704 @default.
• W2545286687 hasConceptScore W2545286687C85789140 @default.
• W2545286687 hasConceptScore W2545286687C86803240 @default.
• W2545286687 hasConceptScore W2545286687C92488253 @default.
• W2545286687 hasConceptScore W2545286687C95444343 @default.

• next