FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2545403780> ?p ?o ?g. }

• W2545403780 abstract "Correct identification of the amyloidosis-causing protein is crucial for clinical management. Recently the Mayo Clinic reported laser-capture microdissection (LCM) with liquid chromatography-coupled tandem mass spectrometry (MS/MS) as a new diagnostic tool for amyloid diagnosis. Here, we report an independent implementation of this proteomic diagnostics method at the Princess Alexandra Hospital Amyloidosis Centre in Brisbane, Australia. From 2010 to 2014, 138 biopsies received from 35 different organ sites were analysed by LCM-MS/MS using Congo Red staining to visualise amyloid deposits. There was insufficient tissue in the block for LCM for 7 cases. An amyloid forming protein was ultimately identified in 121 out of 131 attempted cases (94 %). Of the 121 successful cases, the Mayo Clinic amyloid proteomic signature (at least two of Serum Amyloid P, ApoE and ApoA4) was detected in 92 (76 %). Low levels of additional amyloid forming proteins were frequently identified with the main amyloid forming protein, which may reflect co-deposition of fibrils. Furthermore, vitronectin and clusterin were frequently identified in our samples. Adding vitronectin to the amyloid signature increases the number of positive cases, suggesting a potential 4th protein for the signature. In terms of clinical impact, amyloid typing by immunohistochemistry was attempted in 88 cases, reported as diagnostic in 39, however, 5 were subsequently revealed by proteomic analysis to be incorrect. Overall, the referring clinician’s diagnosis of amyloid subtype was altered by proteomic analysis in 24 % of cases. While LCM-MS/MS was highly robust in protein identification, clinical information was still required for subtyping, particularly for systemic versus localized amyloidosis. This study reports the independent implementation and evaluation of a proteomics-based diagnostic for amyloidosis subtyping. Our results support LCM-MS/MS as a powerful new diagnostic technique for amyloidosis, but also identified some challenges and further development opportunities." @default.
• W2545403780 created "2016-11-04" @default.
• W2545403780 creator A5022427665 @default.
• W2545403780 creator A5035643017 @default.
• W2545403780 creator A5055233024 @default.
• W2545403780 creator A5070498464 @default.
• W2545403780 creator A5076262592 @default.
• W2545403780 creator A5083848941 @default.
• W2545403780 creator A5084858618 @default.
• W2545403780 creator A5091163780 @default.
• W2545403780 date "2016-10-27" @default.
• W2545403780 modified "2023-10-17" @default.
• W2545403780 title "Implementation and evaluation of amyloidosis subtyping by laser-capture microdissection and tandem mass spectrometry" @default.
• W2545403780 cites W1965928109 @default.
• W2545403780 cites W1978083401 @default.
• W2545403780 cites W2010201521 @default.
• W2545403780 cites W2027966577 @default.
• W2545403780 cites W2039881612 @default.
• W2545403780 cites W2046882226 @default.
• W2545403780 cites W2066598200 @default.
• W2545403780 cites W2076467891 @default.
• W2545403780 cites W2090078011 @default.
• W2545403780 cites W2134947970 @default.
• W2545403780 cites W2148158983 @default.
• W2545403780 cites W2155650008 @default.
• W2545403780 cites W2161648900 @default.
• W2545403780 cites W2327237368 @default.
• W2545403780 cites W4210576276 @default.
• W2545403780 cites W4240572343 @default.
• W2545403780 doi "https://doi.org/10.1186/s12014-016-9133-x" @default.
• W2545403780 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5081679" @default.
• W2545403780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27795698" @default.
• W2545403780 hasPublicationYear "2016" @default.
• W2545403780 type Work @default.
• W2545403780 sameAs 2545403780 @default.
• W2545403780 citedByCount "55" @default.
• W2545403780 countsByYear W25454037802017 @default.
• W2545403780 countsByYear W25454037802018 @default.
• W2545403780 countsByYear W25454037802019 @default.
• W2545403780 countsByYear W25454037802020 @default.
• W2545403780 countsByYear W25454037802021 @default.
• W2545403780 countsByYear W25454037802022 @default.
• W2545403780 countsByYear W25454037802023 @default.
• W2545403780 crossrefType "journal-article" @default.
• W2545403780 hasAuthorship W2545403780A5022427665 @default.
• W2545403780 hasAuthorship W2545403780A5035643017 @default.
• W2545403780 hasAuthorship W2545403780A5055233024 @default.
• W2545403780 hasAuthorship W2545403780A5070498464 @default.
• W2545403780 hasAuthorship W2545403780A5076262592 @default.
• W2545403780 hasAuthorship W2545403780A5083848941 @default.
• W2545403780 hasAuthorship W2545403780A5084858618 @default.
• W2545403780 hasAuthorship W2545403780A5091163780 @default.
• W2545403780 hasBestOaLocation W25454037801 @default.
• W2545403780 hasConcept C104317684 @default.
• W2545403780 hasConcept C11242284 @default.
• W2545403780 hasConcept C125965507 @default.
• W2545403780 hasConcept C126322002 @default.
• W2545403780 hasConcept C142724271 @default.
• W2545403780 hasConcept C150194340 @default.
• W2545403780 hasConcept C162356407 @default.
• W2545403780 hasConcept C164144092 @default.
• W2545403780 hasConcept C170493617 @default.
• W2545403780 hasConcept C185592680 @default.
• W2545403780 hasConcept C195687474 @default.
• W2545403780 hasConcept C199360897 @default.
• W2545403780 hasConcept C2777633098 @default.
• W2545403780 hasConcept C2779951007 @default.
• W2545403780 hasConcept C31827203 @default.
• W2545403780 hasConcept C41008148 @default.
• W2545403780 hasConcept C43617362 @default.
• W2545403780 hasConcept C46111723 @default.
• W2545403780 hasConcept C55493867 @default.
• W2545403780 hasConcept C70721500 @default.
• W2545403780 hasConcept C71924100 @default.
• W2545403780 hasConcept C83852419 @default.
• W2545403780 hasConcept C86803240 @default.
• W2545403780 hasConceptScore W2545403780C104317684 @default.
• W2545403780 hasConceptScore W2545403780C11242284 @default.
• W2545403780 hasConceptScore W2545403780C125965507 @default.
• W2545403780 hasConceptScore W2545403780C126322002 @default.
• W2545403780 hasConceptScore W2545403780C142724271 @default.
• W2545403780 hasConceptScore W2545403780C150194340 @default.
• W2545403780 hasConceptScore W2545403780C162356407 @default.
• W2545403780 hasConceptScore W2545403780C164144092 @default.
• W2545403780 hasConceptScore W2545403780C170493617 @default.
• W2545403780 hasConceptScore W2545403780C185592680 @default.
• W2545403780 hasConceptScore W2545403780C195687474 @default.
• W2545403780 hasConceptScore W2545403780C199360897 @default.
• W2545403780 hasConceptScore W2545403780C2777633098 @default.
• W2545403780 hasConceptScore W2545403780C2779951007 @default.
• W2545403780 hasConceptScore W2545403780C31827203 @default.
• W2545403780 hasConceptScore W2545403780C41008148 @default.
• W2545403780 hasConceptScore W2545403780C43617362 @default.
• W2545403780 hasConceptScore W2545403780C46111723 @default.
• W2545403780 hasConceptScore W2545403780C55493867 @default.
• W2545403780 hasConceptScore W2545403780C70721500 @default.
• W2545403780 hasConceptScore W2545403780C71924100 @default.
• W2545403780 hasConceptScore W2545403780C83852419 @default.
• W2545403780 hasConceptScore W2545403780C86803240 @default.
• W2545403780 hasFunder F4320320390 @default.

• next