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- W2545930451 abstract "We demonstrate the first biosensing strategy that relies on quantum dot (QD) fluorescence blinking to report the presence of a target molecule. Unlike other biosensors that utilize QDs, our method does not require the analyte to induce any fluorescence intensity or color changes, making it readily applicable to a wide range of target species. Instead, our approach relies on the understanding that blinking, a single particle phenomenon, is obscured when several QDs lie within the detection volume of a confocal microscope. If QDs are engineered to aggregate when they encounter a particular target molecule, the observation of quasi-continuous emission should indicate its presence. As proof of concept, we programmed DNAs to drive rapid isothermal assembly of QDs in the presence of a target strand (oncogene K-ras). The assemblies, confirmed by various gel techniques, contained multiple QDs and were readily distinguished from free QDs by the absence of blinking." @default.
- W2545930451 created "2016-11-04" @default.
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- W2545930451 date "2016-11-10" @default.
- W2545930451 modified "2023-09-26" @default.
- W2545930451 title "Fluorescence Blinking as an Output Signal for Biosensing" @default.
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- W2545930451 doi "https://doi.org/10.1021/acssensors.6b00352" @default.
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