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- W2546682273 abstract "Sterilized soil is often used as a control to distinguish between biotic and abiotic reactions and in petroleum-contaminated soils it is used to verify bioremediation experiments. In the present study we performed a laboratory and a greenhouse experiment to evaluate the effect of steam sterilization, e.g. autoclaving, on some of the chemical properties of a petroleum-contaminated soil as well the effects on shoot and root growth of maize (Zea mays L.). The results showed that the autoclaving significantly decreased pH and increased the amounts of electrical conductivity and available phosphorus in both contaminated and uncontaminated soils. However total nitrogen, organic carbon and cation exchangeable capacity statistically remained constant. Sterilization also decreased total Zn, Ni and Mn in uncontaminated soil and total Fe in petroleum contaminated soil. Almost all polycyclic aromatic hydrocarbons (PAHs) were strongly increased in contaminated sterilized soil in compared to the non-sterilized one. The total concentration of PAHs increased around 250% as affected by sterilization treatment, while the content of total petroleum hydrocarbon (TPH) in the sterile soil was almost similar to the non-sterile soil. Growing parameters of maize such as root and shoot biomass, shoot height, total root length, number of root tips and root depth were strongly decreased in the contaminated soil. Furthermore sterilization of uncontaminated soil had a positive effect on shoot biomass while it was slightly negative for the contaminated soil. Shoot dry weight of maize planted in uncontaminated sterile soil was about 30% higher than in uncontaminated nonsterile soil. Therefore it is important to consider both the changes of soil properties and petroleum compounds and effects of these alterations on the plant growth when studying autoclaving sterilized soils. K e y w o r d s" @default.
- W2546682273 created "2016-11-11" @default.
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- W2546682273 date "2015-01-01" @default.
- W2546682273 modified "2023-09-23" @default.
- W2546682273 title "The effect of steam sterilization of a petroleum-contaminated soil on PAH concentration and maize (Zea mays L.) growth." @default.
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