Matches in SemOpenAlex for { <https://semopenalex.org/work/W2546985353> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2546985353 endingPage "142" @default.
- W2546985353 startingPage "136" @default.
- W2546985353 abstract "Cutaneous leishmaniasis is a common skin disease caused by leishmania parasite. An accurate diagnosis of parasites species is possible using molecular techniques. This study was carried out to compare internal transcribed spacer (ITS1) and kinetoplast deoxyribonucleic acid (KDNA) genes for identifying Leishmania species by Polymerase Chain Reaction (PCR), furthermore, genetic diversity of isolates was studied. This research examined 130 patients who were suspected of cutaneous leishmaniasis and referred to Kashan's health centers from 2011-2014. After DNA extraction from serosity, PCR were performed using ITS1 and KDNA primers. Cutaneous Leishmaniasis was diagnosed by the observation of 320 bp band in the ITS1-PCR. The PCR products were digested with restriction enzyme HaeIII and then leishmania species were identified by patterns of enzymatic digestion. The diagnostic criteria of Cutaneous Leishmaniasis (CL) in KDNA-PCR were based on the observation of 760 and 650 bp for Leishmaniasis tropica and Leishmaniasis major, respectively. Twelve isolates of leishmania were sequenced and the phylogenetic tree was traced using the results of sequencing by Mega 4 software. Out of 130 suspected patients to CL, 70 (53.8%) and 98 (75.4%) isolates were positive by Restriction Fragment Length Polymorphism (RFLP) of ITS1 and KDNA, respectively. Using ITS1 PCR, 60 samples (85.7%) and 10 samples (14.3%) were identified as L. tropica and L. major, respectively, ITS1-PCR had 25.3% false negative, compare to microscopy. While, microscopy had false negative in 13 cases compare to KDNA-PCR. Due to the lower sensitivity of the PCR-RFLP of ITS1, KDNA-PCR is recommended for diagnosis of CL. The L. tropica and L. major are the causative agents of CL." @default.
- W2546985353 created "2016-11-11" @default.
- W2546985353 creator A5004329191 @default.
- W2546985353 creator A5017626982 @default.
- W2546985353 creator A5021744010 @default.
- W2546985353 creator A5086830256 @default.
- W2546985353 date "2016-03-15" @default.
- W2546985353 modified "2023-09-27" @default.
- W2546985353 title "Molecular Diagnosis of Clinical Isolates of Cutaneous Leishmaniasis Using ITS1 and KDNA Genes and Genetic Polymorphism of Leishmania in Kashan, Iran" @default.
- W2546985353 cites W1999238025 @default.
- W2546985353 cites W2001702414 @default.
- W2546985353 cites W2022424663 @default.
- W2546985353 cites W2030863474 @default.
- W2546985353 cites W2040598188 @default.
- W2546985353 cites W2074700063 @default.
- W2546985353 cites W2086042583 @default.
- W2546985353 cites W2086074501 @default.
- W2546985353 cites W2159804482 @default.
- W2546985353 cites W2162623444 @default.
- W2546985353 cites W2167835414 @default.
- W2546985353 cites W2168277165 @default.
- W2546985353 cites W2170956456 @default.
- W2546985353 cites W2171190168 @default.
- W2546985353 cites W4211078476 @default.
- W2546985353 doi "https://doi.org/10.3923/pjbs.2016.136.142" @default.
- W2546985353 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29023050" @default.
- W2546985353 hasPublicationYear "2016" @default.
- W2546985353 type Work @default.
- W2546985353 sameAs 2546985353 @default.
- W2546985353 citedByCount "7" @default.
- W2546985353 countsByYear W25469853532018 @default.
- W2546985353 countsByYear W25469853532019 @default.
- W2546985353 countsByYear W25469853532021 @default.
- W2546985353 countsByYear W25469853532022 @default.
- W2546985353 crossrefType "journal-article" @default.
- W2546985353 hasAuthorship W2546985353A5004329191 @default.
- W2546985353 hasAuthorship W2546985353A5017626982 @default.
- W2546985353 hasAuthorship W2546985353A5021744010 @default.
- W2546985353 hasAuthorship W2546985353A5086830256 @default.
- W2546985353 hasBestOaLocation W25469853531 @default.
- W2546985353 hasConcept C104317684 @default.
- W2546985353 hasConcept C128319531 @default.
- W2546985353 hasConcept C136764020 @default.
- W2546985353 hasConcept C159047783 @default.
- W2546985353 hasConcept C168007829 @default.
- W2546985353 hasConcept C2776027055 @default.
- W2546985353 hasConcept C2776555147 @default.
- W2546985353 hasConcept C2778338367 @default.
- W2546985353 hasConcept C2778702967 @default.
- W2546985353 hasConcept C2780599195 @default.
- W2546985353 hasConcept C2781092759 @default.
- W2546985353 hasConcept C41008148 @default.
- W2546985353 hasConcept C49105822 @default.
- W2546985353 hasConcept C54355233 @default.
- W2546985353 hasConcept C552990157 @default.
- W2546985353 hasConcept C71928629 @default.
- W2546985353 hasConcept C78063203 @default.
- W2546985353 hasConcept C86803240 @default.
- W2546985353 hasConceptScore W2546985353C104317684 @default.
- W2546985353 hasConceptScore W2546985353C128319531 @default.
- W2546985353 hasConceptScore W2546985353C136764020 @default.
- W2546985353 hasConceptScore W2546985353C159047783 @default.
- W2546985353 hasConceptScore W2546985353C168007829 @default.
- W2546985353 hasConceptScore W2546985353C2776027055 @default.
- W2546985353 hasConceptScore W2546985353C2776555147 @default.
- W2546985353 hasConceptScore W2546985353C2778338367 @default.
- W2546985353 hasConceptScore W2546985353C2778702967 @default.
- W2546985353 hasConceptScore W2546985353C2780599195 @default.
- W2546985353 hasConceptScore W2546985353C2781092759 @default.
- W2546985353 hasConceptScore W2546985353C41008148 @default.
- W2546985353 hasConceptScore W2546985353C49105822 @default.
- W2546985353 hasConceptScore W2546985353C54355233 @default.
- W2546985353 hasConceptScore W2546985353C552990157 @default.
- W2546985353 hasConceptScore W2546985353C71928629 @default.
- W2546985353 hasConceptScore W2546985353C78063203 @default.
- W2546985353 hasConceptScore W2546985353C86803240 @default.
- W2546985353 hasIssue "3" @default.
- W2546985353 hasLocation W25469853531 @default.
- W2546985353 hasLocation W25469853532 @default.
- W2546985353 hasOpenAccess W2546985353 @default.
- W2546985353 hasPrimaryLocation W25469853531 @default.
- W2546985353 hasRelatedWork W1999238025 @default.
- W2546985353 hasRelatedWork W2003044652 @default.
- W2546985353 hasRelatedWork W2018402366 @default.
- W2546985353 hasRelatedWork W2074700063 @default.
- W2546985353 hasRelatedWork W2078526653 @default.
- W2546985353 hasRelatedWork W2159182616 @default.
- W2546985353 hasRelatedWork W2260073393 @default.
- W2546985353 hasRelatedWork W2290505432 @default.
- W2546985353 hasRelatedWork W2546985353 @default.
- W2546985353 hasRelatedWork W2946670007 @default.
- W2546985353 hasVolume "19" @default.
- W2546985353 isParatext "false" @default.
- W2546985353 isRetracted "false" @default.
- W2546985353 magId "2546985353" @default.
- W2546985353 workType "article" @default.