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• W2547207805 abstract "In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine (as a spacer arm). The p(GMA) grafted and SA modified form (i.e., Fe3O4@SiO2@pGMA-SA-3) was used for covalent immobilization of invertase (EC 3.2.1.26). The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA)-SA-3 was 36.1 ± 0.9 and 33.4 ± 1.3 mg/g, respectively. The Km and Vmax values of immobilized invertase were found to be 39.4 mmol/L and 349.5 mmol/L min, and not significantly changed compared with free form (34.3 mmol/L and 387.2 mmol/L min), respectively, revealed that the applied protocol did not have any detrimental effect on the retained activity of immobilized invertase." @default.
• W2547207805 created "2016-11-11" @default.
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• W2547207805 date "2017-04-01" @default.
• W2547207805 modified "2023-10-06" @default.
• W2547207805 title "Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles" @default.
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• W2547207805 doi "https://doi.org/10.1016/j.foodchem.2016.11.007" @default.
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