Matches in SemOpenAlex for { <https://semopenalex.org/work/W254787856> ?p ?o ?g. }
Showing items 1 to 55 of
55
with 100 items per page.
- W254787856 abstract "MYB genes encode a large family of transcription regulators in eukaryotes. Most plant MYB genes belong to the R2R3-MYB subfamily. MYB gene family members have been studied in various plant species and play roles in the regulation of plant development, hormone signalling, defence response and secondary metabolism. Orchid plants play an important role in the floral industry owing to their unique flower shapes, fragrance, colours and keeping quality. Flower colours are influenced by flower pigmentation, vacuolar compounds, pH, metal ions and shape of epidermal cells. R2R3-MYB genes regulate flower pigmentation, flower shape and cell shape therefore have importance in the development of new varieties of orchids. Polymerase chain reaction with sequence consensus based primer was used to amplify thirty-seven partial cDNA fragments representing R2R3-MYB gene sequences were isolated from Dendrobium hybrida. Phylogenetic analysis with IT3F: An Interspecies Transcription Factor Function Finder for Plants (http://jicbio.bbsrc.ac.uk/IT3F) of the translated R2R3-MYB gene sequences revealed that MYB sequences from Dendrobium hybrida are clustered into six subgroups. Nine of the translated DhMYB genes were clustered in subgroup 9 which are known to be involved in cell shape development. Production of dsRNA, sense and antisense RNAs were synthesized in vitro from the corresponding DNA plasmids by using double T7 RNA polymerase. RNAi knock down of the expression of DhMYB using dsRNA approach showed significant reduction of DhMYB gene expression compared with untreated control. Cell shape scanning electron microscopy of adaxial epidermal cells within domain 1 of the lip of flowers treated with DhMYB dsRNA had flattened epidermal cells whilst those of control flowers were conical – papillate. We suggest that DhMYB cDNA controls cell morphogenesis in the lip epidermis." @default.
- W254787856 created "2016-06-24" @default.
- W254787856 creator A5077499270 @default.
- W254787856 date "2013-01-01" @default.
- W254787856 modified "2023-09-27" @default.
- W254787856 title "An investigation of the roles of MYB transcription factors in orchid dendrobium hybrida flower development / Lau Su Ee" @default.
- W254787856 hasPublicationYear "2013" @default.
- W254787856 type Work @default.
- W254787856 sameAs 254787856 @default.
- W254787856 citedByCount "0" @default.
- W254787856 crossrefType "dissertation" @default.
- W254787856 hasAuthorship W254787856A5077499270 @default.
- W254787856 hasConcept C104317684 @default.
- W254787856 hasConcept C2776219560 @default.
- W254787856 hasConcept C50929876 @default.
- W254787856 hasConcept C54355233 @default.
- W254787856 hasConcept C59822182 @default.
- W254787856 hasConcept C62794011 @default.
- W254787856 hasConcept C86339819 @default.
- W254787856 hasConcept C86803240 @default.
- W254787856 hasConceptScore W254787856C104317684 @default.
- W254787856 hasConceptScore W254787856C2776219560 @default.
- W254787856 hasConceptScore W254787856C50929876 @default.
- W254787856 hasConceptScore W254787856C54355233 @default.
- W254787856 hasConceptScore W254787856C59822182 @default.
- W254787856 hasConceptScore W254787856C62794011 @default.
- W254787856 hasConceptScore W254787856C86339819 @default.
- W254787856 hasConceptScore W254787856C86803240 @default.
- W254787856 hasLocation W2547878561 @default.
- W254787856 hasOpenAccess W254787856 @default.
- W254787856 hasPrimaryLocation W2547878561 @default.
- W254787856 hasRelatedWork W1801492160 @default.
- W254787856 hasRelatedWork W2022363784 @default.
- W254787856 hasRelatedWork W2022596175 @default.
- W254787856 hasRelatedWork W2058108065 @default.
- W254787856 hasRelatedWork W2059137444 @default.
- W254787856 hasRelatedWork W2073431197 @default.
- W254787856 hasRelatedWork W2079284215 @default.
- W254787856 hasRelatedWork W2095136479 @default.
- W254787856 hasRelatedWork W2104410485 @default.
- W254787856 hasRelatedWork W2117989671 @default.
- W254787856 hasRelatedWork W2119564723 @default.
- W254787856 hasRelatedWork W2119749921 @default.
- W254787856 hasRelatedWork W2129777440 @default.
- W254787856 hasRelatedWork W2166436705 @default.
- W254787856 hasRelatedWork W2341575173 @default.
- W254787856 hasRelatedWork W2417746187 @default.
- W254787856 hasRelatedWork W3000524752 @default.
- W254787856 hasRelatedWork W3038784166 @default.
- W254787856 hasRelatedWork W3044163762 @default.
- W254787856 hasRelatedWork W3164450208 @default.
- W254787856 isParatext "false" @default.
- W254787856 isRetracted "false" @default.
- W254787856 magId "254787856" @default.
- W254787856 workType "dissertation" @default.