FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2548008859> ?p ?o ?g. }

• W2548008859 abstract "ABSTRACT Human TIM and TAM family proteins were recently found to serve as phosphatidylserine (PS) receptors which promote infections by many different viruses, including dengue virus, West Nile virus, Ebola virus, Marburg virus, and Zika virus. In the present study, we provide substantial evidence demonstrating that TIM-1 is important for efficient infection by hepatitis C virus (HCV). The knockdown of TIM-1 expression significantly reduced HCV infection but not HCV RNA replication. Likewise, TIM-1 knockout in Huh-7.5 cells remarkably lowered HCV cell attachment and subsequent HCV infection. More significantly, the impairment of HCV infection in the TIM-1 knockout cells could be restored completely by ectopic expression of TIM-1 but not TIM-3 or TIM-4. Additionally, HCV infection and cell attachment were inhibited by PS but not by phosphatidylcholine (PC), demonstrating that TIM-1-mediated enhancement of HCV infection is PS dependent. The exposure of PS on the HCV envelope was confirmed by immunoprecipitation of HCV particles with a PS-specific monoclonal antibody. Collectively, these findings demonstrate that TIM-1 promotes HCV infection by serving as an attachment receptor for binding to PS exposed on the HCV envelope. IMPORTANCE TIM family proteins were recently found to enhance infections by many different viruses, including several members of the Flaviviridae family. However, their importance in HCV infection has not previously been examined experimentally. The TIM family proteins include three members in humans: TIM-1, TIM-3, and TIM-4. The findings derived from our studies demonstrate that TIM-1, but not TIM-3 or TIM-4, promotes HCV infection by functioning as an HCV attachment factor. Knockout of the TIM-1 gene resulted in a remarkable reduction of HCV cell attachment and infection. PS-containing liposomes blocked HCV cell attachment and subsequent HCV infection. HCV particles could also be precipitated with a PS-specific monoclonal antibody. These findings suggest that TIM-1 and its binding ligand, PS, may serve as novel targets for antiviral intervention." @default.
• W2548008859 created "2016-11-11" @default.
• W2548008859 creator A5032891751 @default.
• W2548008859 creator A5037677450 @default.
• W2548008859 creator A5051406306 @default.
• W2548008859 creator A5065592955 @default.
• W2548008859 date "2017-01-15" @default.
• W2548008859 modified "2023-10-17" @default.
• W2548008859 title "TIM-1 Promotes Hepatitis C Virus Cell Attachment and Infection" @default.
• W2548008859 cites W1857453373 @default.
• W2548008859 cites W1968376947 @default.
• W2548008859 cites W1970362643 @default.
• W2548008859 cites W1971505503 @default.
• W2548008859 cites W1977889013 @default.
• W2548008859 cites W1978224572 @default.
• W2548008859 cites W1981955271 @default.
• W2548008859 cites W1984595411 @default.
• W2548008859 cites W1986505810 @default.
• W2548008859 cites W1987287179 @default.
• W2548008859 cites W1988656403 @default.
• W2548008859 cites W1992310739 @default.
• W2548008859 cites W2004817638 @default.
• W2548008859 cites W2006309458 @default.
• W2548008859 cites W2006524544 @default.
• W2548008859 cites W2008483588 @default.
• W2548008859 cites W2010579714 @default.
• W2548008859 cites W2015489330 @default.
• W2548008859 cites W2022687771 @default.
• W2548008859 cites W2023402045 @default.
• W2548008859 cites W2025124945 @default.
• W2548008859 cites W2026051181 @default.
• W2548008859 cites W2030618002 @default.
• W2548008859 cites W2033110360 @default.
• W2548008859 cites W2034241642 @default.
• W2548008859 cites W2046182783 @default.
• W2548008859 cites W2050028384 @default.
• W2548008859 cites W2051481258 @default.
• W2548008859 cites W2057967679 @default.
• W2548008859 cites W2064526034 @default.
• W2548008859 cites W2065473039 @default.
• W2548008859 cites W2066203226 @default.
• W2548008859 cites W2071776498 @default.
• W2548008859 cites W2071997899 @default.
• W2548008859 cites W2072904045 @default.
• W2548008859 cites W2075147482 @default.
• W2548008859 cites W2084858225 @default.
• W2548008859 cites W2092012609 @default.
• W2548008859 cites W2096347191 @default.
• W2548008859 cites W2096413300 @default.
• W2548008859 cites W2100768492 @default.
• W2548008859 cites W2100943457 @default.
• W2548008859 cites W2105291849 @default.
• W2548008859 cites W2122121966 @default.
• W2548008859 cites W2122756910 @default.
• W2548008859 cites W2124339485 @default.
• W2548008859 cites W2126772900 @default.
• W2548008859 cites W2131586101 @default.
• W2548008859 cites W2133588441 @default.
• W2548008859 cites W2135336711 @default.
• W2548008859 cites W2146730705 @default.
• W2548008859 cites W2151898297 @default.
• W2548008859 cites W2155431764 @default.
• W2548008859 cites W2160174559 @default.
• W2548008859 cites W2161906796 @default.
• W2548008859 cites W2167717479 @default.
• W2548008859 cites W2169112650 @default.
• W2548008859 doi "https://doi.org/10.1128/jvi.01583-16" @default.
• W2548008859 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5215352" @default.
• W2548008859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27807228" @default.
• W2548008859 hasPublicationYear "2017" @default.
• W2548008859 type Work @default.
• W2548008859 sameAs 2548008859 @default.
• W2548008859 citedByCount "22" @default.
• W2548008859 countsByYear W25480088592017 @default.
• W2548008859 countsByYear W25480088592018 @default.
• W2548008859 countsByYear W25480088592019 @default.
• W2548008859 countsByYear W25480088592020 @default.
• W2548008859 countsByYear W25480088592021 @default.
• W2548008859 countsByYear W25480088592022 @default.
• W2548008859 countsByYear W25480088592023 @default.
• W2548008859 crossrefType "journal-article" @default.
• W2548008859 hasAuthorship W2548008859A5032891751 @default.
• W2548008859 hasAuthorship W2548008859A5037677450 @default.
• W2548008859 hasAuthorship W2548008859A5051406306 @default.
• W2548008859 hasAuthorship W2548008859A5065592955 @default.
• W2548008859 hasBestOaLocation W25480088591 @default.
• W2548008859 hasConcept C140704245 @default.
• W2548008859 hasConcept C159047783 @default.
• W2548008859 hasConcept C196594336 @default.
• W2548008859 hasConcept C2522874641 @default.
• W2548008859 hasConcept C2776408679 @default.
• W2548008859 hasConcept C2777510210 @default.
• W2548008859 hasConcept C2779635636 @default.
• W2548008859 hasConcept C2780568436 @default.
• W2548008859 hasConcept C58475186 @default.
• W2548008859 hasConcept C86803240 @default.
• W2548008859 hasConcept C97380033 @default.
• W2548008859 hasConceptScore W2548008859C140704245 @default.
• W2548008859 hasConceptScore W2548008859C159047783 @default.
• W2548008859 hasConceptScore W2548008859C196594336 @default.

• next