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- W2548013886 abstract "Multiple shoots were induced from the shoot tip explants derivedfrom the in vitro grown seedlings of an endangered and horticulturalimportant epiphyte orchid, Dimorphorchis lowii. Shoot tip explantswere cultured vertically and/or horizontally on solidified Knudson Cmedia (KC) added with various concentrations of Kinetin (Kn) and 6-Benzylaminopurine (BAP) for shoots multiplication. Shoots wereinitiated after 4 weeks of culture, and the highest number of healthyshoots (5.05 shoots per explant) was observed in 2.0 mg/l Kinetin(Kn), when the explant placed horizontally. Regenerated shoots wereroot-induced in KC medium with various concentrations andcombinations of Naphthalene acetic acid (NAA), Indole acetic acid(IAA) and Indole butyric acid (IBA). Shoots cultured on mediumwith 1.0 mg/l IAA and 0.5 mg/l IBA was the most appropriatecombination for rooting. Rooted plantlets were transferred in amedium mixture containing coco peat and sphagnum moss (2:1).After 2 months, 78% of plants survived when transferred to theglasshouse. This is the first report for in vitro propagation of D. lowiithrough shoot tip culture. The protocol developed can be utilized forboth large-scale plant production and germplasm conservation of thisspecies" @default.
- W2548013886 created "2016-11-11" @default.
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- W2548013886 date "2016-08-13" @default.
- W2548013886 modified "2023-09-23" @default.
- W2548013886 title "Effect of Growth Regulators and Explant Orientation on Shoot Tip Culture of Borneo Endemic Orchid, Dimorphorchis lowii" @default.
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