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• W2548238468 endingPage "145" @default.
• W2548238468 startingPage "140" @default.
• W2548238468 abstract "Recombinant therapeutic monoclonal antibodies (mAbs) must be purified from host cell proteins (HCPs), DNA, and other impurities present in Chinese hamster ovary (CHO) cell culture media. HCPs can potentially result in adverse clinical responses in patients and, in specific cases, have caused degradation of the final mAb product. As reported previously, residual traces of cathepsin D caused particle formation in the final product of mAb-1. The current work was focused on identification of a primary sequence in mAb-1 responsible for the binding and consequent co-purification of trace levels of CHO cathepsin D. Surface plasmon resonance (SPR) was used to detect binding between immobilized CHO cathepsin D and a panel of mAbs. Out of 13 mAbs tested, only mAb-1 and mAb-6 bound to cathepsin D. An LYY motif in the HC CDR2 was common, yet unique, to only these two mAbs. Mutation of LYY to AAA eliminated binding of mAb-1 to cathepsin D providing confirmation that this sequence motif was involved in the binding to CHO cathepsin D. Interestingly, the binding between mAb-1 and cathepsin D was weaker than that of mAb-6, which may be related to the fact that two aspartic acid residues near the LYY motif in mAb-1 are replaced with neutral serine residues in mAb-6. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:140-145, 2017." @default.
• W2548238468 created "2016-11-11" @default.
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• W2548238468 date "2016-11-14" @default.
• W2548238468 modified "2023-10-06" @default.
• W2548238468 title "Identification of an IgG CDR sequence contributing to co-purification of the host cell protease cathepsin D" @default.
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• W2548238468 doi "https://doi.org/10.1002/btpr.2397" @default.
• W2548238468 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27798957" @default.
• W2548238468 hasPublicationYear "2016" @default.
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