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- W2548286242 abstract "The study and use of bioluminescence and chemiluminescence has increased dramatically in the recent years. The applications have extended from ATP-luciferase assay and cell viability tests, to current immunoassay, DNA, genomic, and proteomic analysis. Unlike a fluorescence system, there is no need for an exogenous light source; luminescence is generated from chemical reactions. Utilizing photoncounting detectors, which count the number of photons generated from a chemical reaction, luminescence assay has become one of the most sensitive and quantitative optical detection methods. During the past 15 years, nucleic acid (NA) hybridization techniques have become increasingly important as discovery tools and for the clinical diagnosis of genetic disorders and infectious diseases [1-3] . The application of these methodologies has tremendously profited from the development of nucleic acid amplification techniques that have enormously improved the sensitivity of detection of nucleic acids such as DNA and RNA their chimeric derivatives. This detection method is suited for low abundant gene transcripts and genotyping of rare allele mutations, as well as in the measurement of low copy number RNA and DNA pathogens (bacteria, viruses) in diverse biological sources, especially in applications which depend on extremely limited nucleic acid samples, such as cancer diagnositics, pharmacogenomics, toxicogenomics, forensics, food industry, and environmental detection." @default.
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- W2548286242 date "2007-01-01" @default.
- W2548286242 modified "2023-09-23" @default.
- W2548286242 title "Attomoles Quantitative Chemiluminescence for Molecular Diagnostics" @default.
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