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- W2548366585 abstract "Abstract Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD‐dependent glucose dehydrogenase (GDH) ( Glomorella cingulata ) and bilirubin oxidase (BOx) ( Myrothecium verrucaria ). Glucose dehydrogenase was immobilized by drop‐casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium‐modified NPG electrode. The stability of the bilirubin‐oxidase‐modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx‐modified NPG cathode (500 nm thickness) and a GDH‐modified anode (300 nm), which generated power densities of 17.5 and 7.0 μW cm −2 in phosphate‐buffered saline and artificial serum, respectively." @default.
- W2548366585 created "2016-11-11" @default.
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- W2548366585 date "2016-11-25" @default.
- W2548366585 modified "2023-10-10" @default.
- W2548366585 title "Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells" @default.
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- W2548366585 doi "https://doi.org/10.1002/cplu.201600455" @default.
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