FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2548392913> ?p ?o ?g. }

• W2548392913 endingPage "4145" @default.
• W2548392913 startingPage "4139" @default.
• W2548392913 abstract "BACKGROUND MicroRNA-133b (miR-133b) has been reported to be involved in many diseases, including ovarian cancer and osteosarcoma. Accumulating evidence suggests that miR-133b plays important roles in human disease. In this study, we aimed to investigate the molecular mechanism, including the potential regulator and signaling pathways, of BCL2L2. MATERIAL AND METHODS We first searched the online miRNA database (www.mirdb.org) using the seed sequence located within the 3'-UTR of the target gene, and then performed luciferase assay to test the regulatory relationship between miR-133b and BCL2L2. Western blot and real-time PCR were used to determine the expression of BCL2L2 in human samples or cells treated with miRNA mimics or inhibitors. Flow cytometry was conducted to evaluate the apoptosis status of the cells. RESULTS We validated BCL2L2 to be the direct gene using a luciferase reporter assay. We also conducted real-time PCR and Western blot analyses to study the mRNA and protein expression level of BCL2L2 among different groups (control: n=29, cataract: n=33) or cells treated with scramble control, miR-133b mimics, BCL2L2 siRNA, and miR-133b inhibitors, and identified the negative regulatory relationship between miR-133b and BCL2L2. We also conducted experiments to investigate the influence of miR-133b and BCL2L2 on the viability and apoptosis of cells. The results showed that miR-133b positively interfered with the viability of cells, while BCL2L2 negatively interfered with the viability of cells, and that miR-133b inhibited apoptosis while BCL2L2 accelerated apoptosis. CONCLUSIONS BCL2L2 was the virtual target of miR-133b, and we found a negative regulatory relationship between miR-133b and BCL2L2. MiR-133b and BCL2L2 interfered with the viability and apoptosis of cells." @default.
• W2548392913 created "2016-11-11" @default.
• W2548392913 creator A5039032252 @default.
• W2548392913 creator A5052274720 @default.
• W2548392913 creator A5090846501 @default.
• W2548392913 date "2016-11-01" @default.
• W2548392913 modified "2023-09-27" @default.
• W2548392913 title "Down-Regulation of MicroRNA-133b Suppresses Apoptosis of Lens Epithelial Cell by Up-Regulating BCL2L2 in Age-Related Cataracts" @default.
• W2548392913 cites W129181430 @default.
• W2548392913 cites W144423133 @default.
• W2548392913 cites W1958564668 @default.
• W2548392913 cites W1979322303 @default.
• W2548392913 cites W1983908748 @default.
• W2548392913 cites W1985563073 @default.
• W2548392913 cites W1998798740 @default.
• W2548392913 cites W1999660608 @default.
• W2548392913 cites W2003355295 @default.
• W2548392913 cites W2004329409 @default.
• W2548392913 cites W2011823018 @default.
• W2548392913 cites W2017181541 @default.
• W2548392913 cites W2019705371 @default.
• W2548392913 cites W2024996523 @default.
• W2548392913 cites W2038622868 @default.
• W2548392913 cites W2046109186 @default.
• W2548392913 cites W2059668731 @default.
• W2548392913 cites W2066404900 @default.
• W2548392913 cites W2066640259 @default.
• W2548392913 cites W2067260619 @default.
• W2548392913 cites W2083599686 @default.
• W2548392913 cites W2105214845 @default.
• W2548392913 cites W2105973891 @default.
• W2548392913 cites W2150048195 @default.
• W2548392913 cites W2165456300 @default.
• W2548392913 cites W2168310929 @default.
• W2548392913 cites W2177869125 @default.
• W2548392913 cites W2185567659 @default.
• W2548392913 cites W2256314444 @default.
• W2548392913 cites W2318328899 @default.
• W2548392913 cites W2561069604 @default.
• W2548392913 cites W265833079 @default.
• W2548392913 doi "https://doi.org/10.12659/msm.896975" @default.
• W2548392913 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5094470" @default.
• W2548392913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27802259" @default.
• W2548392913 hasPublicationYear "2016" @default.
• W2548392913 type Work @default.
• W2548392913 sameAs 2548392913 @default.
• W2548392913 citedByCount "22" @default.
• W2548392913 countsByYear W25483929132017 @default.
• W2548392913 countsByYear W25483929132018 @default.
• W2548392913 countsByYear W25483929132019 @default.
• W2548392913 countsByYear W25483929132020 @default.
• W2548392913 countsByYear W25483929132021 @default.
• W2548392913 countsByYear W25483929132022 @default.
• W2548392913 countsByYear W25483929132023 @default.
• W2548392913 crossrefType "journal-article" @default.
• W2548392913 hasAuthorship W2548392913A5039032252 @default.
• W2548392913 hasAuthorship W2548392913A5052274720 @default.
• W2548392913 hasAuthorship W2548392913A5090846501 @default.
• W2548392913 hasBestOaLocation W25483929132 @default.
• W2548392913 hasConcept C104317684 @default.
• W2548392913 hasConcept C145059251 @default.
• W2548392913 hasConcept C153911025 @default.
• W2548392913 hasConcept C190283241 @default.
• W2548392913 hasConcept C2776415932 @default.
• W2548392913 hasConcept C53227056 @default.
• W2548392913 hasConcept C54355233 @default.
• W2548392913 hasConcept C553184892 @default.
• W2548392913 hasConcept C55493867 @default.
• W2548392913 hasConcept C81885089 @default.
• W2548392913 hasConcept C86803240 @default.
• W2548392913 hasConcept C95444343 @default.
• W2548392913 hasConceptScore W2548392913C104317684 @default.
• W2548392913 hasConceptScore W2548392913C145059251 @default.
• W2548392913 hasConceptScore W2548392913C153911025 @default.
• W2548392913 hasConceptScore W2548392913C190283241 @default.
• W2548392913 hasConceptScore W2548392913C2776415932 @default.
• W2548392913 hasConceptScore W2548392913C53227056 @default.
• W2548392913 hasConceptScore W2548392913C54355233 @default.
• W2548392913 hasConceptScore W2548392913C553184892 @default.
• W2548392913 hasConceptScore W2548392913C55493867 @default.
• W2548392913 hasConceptScore W2548392913C81885089 @default.
• W2548392913 hasConceptScore W2548392913C86803240 @default.
• W2548392913 hasConceptScore W2548392913C95444343 @default.
• W2548392913 hasLocation W25483929131 @default.
• W2548392913 hasLocation W25483929132 @default.
• W2548392913 hasLocation W25483929133 @default.
• W2548392913 hasLocation W25483929134 @default.
• W2548392913 hasOpenAccess W2548392913 @default.
• W2548392913 hasPrimaryLocation W25483929131 @default.
• W2548392913 hasRelatedWork W2253642286 @default.
• W2548392913 hasRelatedWork W2351699250 @default.
• W2548392913 hasRelatedWork W2354594137 @default.
• W2548392913 hasRelatedWork W2360919160 @default.
• W2548392913 hasRelatedWork W2380401997 @default.
• W2548392913 hasRelatedWork W2387700717 @default.
• W2548392913 hasRelatedWork W2390532320 @default.
• W2548392913 hasRelatedWork W2392942585 @default.
• W2548392913 hasRelatedWork W2393271317 @default.

• next