FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2548653394> ?p ?o ?g. }

• W2548653394 abstract "Background The study is inclined to investigate the antagonistic effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenovirus (Ad-hENDO-VEGI) on homocysteine (Hcy)-induced vascular endothelial cells (VECs) injury in vitro and in vivo. Methods Human VECs cell line ECV304 was selected and infected with Ad-hENDO-VEGI. The LDH leakage, SOD activity, and MDA levels were measured by the automatic biochemical analyzer. Cell survival rate was counted by Trypanblau dying. The TNF-α and MCP-1 protein expressions were detected by ELISA assay. The protein expressions of fusion protein of Ad-hENDO-VEGI, nuclear factor kappa B p65 (NF-kappa B p65), and NF-kappa B inhibitor alpha (I-kappa B-α) were detected by Western blotting. A rat model of hyper-homocysteinemia was constructed. Thirty-six Wistar rats were randomly divided into 3 groups: the control group, the model group, and the Ad-hENDO-VEGI group. Serum Hcy levels in rats were measured with enzymatic cycling method. Endothelial vasodilation function was evaluated with the treatment of sodium nitroprusside and acetylcholine. Results After Ad-hENDO-VEGI infection, high expressions (41 kD) of fusion proteins in ECV304 cells were observed. The injury severity of Hcy on ECV304 cells had a dose-dependent manner, and the injury reached a steady stage at 1.0 mmol/L. Thus, 1.0 mmol/L Hcy was selected for further experiments. With an increase of Ad-hENDO-VEGI in ECV304 cells after Hcy treatment, LDH leakage, MDA, TNF-α, MCP-1, and nuclear NF-kappa B p65 protein expression were gradually decreased, and cell survival rate, SOD activity, and I-kappa B-α protein expression were gradually increased. Compared with the control group, the model group had a higher Hcy level and attenuated vasodilator response. The Ad-hENDO-VEGI group exhibited a lower Hcy level and enhanced vasodilator response than the model group. Conclusion These results indicated that Ad-hENDO-VEGI could down-regulate NF-kappa B p65 expression and suppress inflammatory response, thereby alleviating Hcy-induced VECs injury." @default.
• W2548653394 created "2016-11-11" @default.
• W2548653394 creator A5056863910 @default.
• W2548653394 creator A5062573587 @default.
• W2548653394 creator A5083476431 @default.
• W2548653394 date "2016-11-01" @default.
• W2548653394 modified "2023-10-02" @default.
• W2548653394 title "Antagonistic effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenovirus on homocysteine-induced vascular endothelial cells injury in vitro and in vivo" @default.
• W2548653394 cites W1598408991 @default.
• W2548653394 cites W165670055 @default.
• W2548653394 cites W1972288532 @default.
• W2548653394 cites W1984280786 @default.
• W2548653394 cites W1987572909 @default.
• W2548653394 cites W1988194907 @default.
• W2548653394 cites W1994342925 @default.
• W2548653394 cites W2002942232 @default.
• W2548653394 cites W2017699378 @default.
• W2548653394 cites W2041843882 @default.
• W2548653394 cites W2045096300 @default.
• W2548653394 cites W2046159681 @default.
• W2548653394 cites W2073303759 @default.
• W2548653394 cites W2079690131 @default.
• W2548653394 cites W2081863867 @default.
• W2548653394 cites W2095131091 @default.
• W2548653394 cites W2108181896 @default.
• W2548653394 cites W2146672236 @default.
• W2548653394 cites W2152573307 @default.
• W2548653394 cites W2153667577 @default.
• W2548653394 cites W2160851275 @default.
• W2548653394 cites W2215788469 @default.
• W2548653394 cites W2279841845 @default.
• W2548653394 cites W2319393335 @default.
• W2548653394 cites W2338875178 @default.
• W2548653394 doi "https://doi.org/10.1097/md.0000000000005197" @default.
• W2548653394 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5591108" @default.
• W2548653394 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27858860" @default.
• W2548653394 hasPublicationYear "2016" @default.
• W2548653394 type Work @default.
• W2548653394 sameAs 2548653394 @default.
• W2548653394 citedByCount "2" @default.
• W2548653394 countsByYear W25486533942017 @default.
• W2548653394 countsByYear W25486533942020 @default.
• W2548653394 crossrefType "journal-article" @default.
• W2548653394 hasAuthorship W2548653394A5056863910 @default.
• W2548653394 hasAuthorship W2548653394A5062573587 @default.
• W2548653394 hasAuthorship W2548653394A5083476431 @default.
• W2548653394 hasBestOaLocation W25486533941 @default.
• W2548653394 hasConcept C104317684 @default.
• W2548653394 hasConcept C123012128 @default.
• W2548653394 hasConcept C123894998 @default.
• W2548653394 hasConcept C126322002 @default.
• W2548653394 hasConcept C146285616 @default.
• W2548653394 hasConcept C150710994 @default.
• W2548653394 hasConcept C150903083 @default.
• W2548653394 hasConcept C153911025 @default.
• W2548653394 hasConcept C167734588 @default.
• W2548653394 hasConcept C17991360 @default.
• W2548653394 hasConcept C202751555 @default.
• W2548653394 hasConcept C203014093 @default.
• W2548653394 hasConcept C207001950 @default.
• W2548653394 hasConcept C2776992346 @default.
• W2548653394 hasConcept C2777025900 @default.
• W2548653394 hasConcept C40767141 @default.
• W2548653394 hasConcept C55493867 @default.
• W2548653394 hasConcept C71924100 @default.
• W2548653394 hasConcept C86803240 @default.
• W2548653394 hasConcept C98274493 @default.
• W2548653394 hasConceptScore W2548653394C104317684 @default.
• W2548653394 hasConceptScore W2548653394C123012128 @default.
• W2548653394 hasConceptScore W2548653394C123894998 @default.
• W2548653394 hasConceptScore W2548653394C126322002 @default.
• W2548653394 hasConceptScore W2548653394C146285616 @default.
• W2548653394 hasConceptScore W2548653394C150710994 @default.
• W2548653394 hasConceptScore W2548653394C150903083 @default.
• W2548653394 hasConceptScore W2548653394C153911025 @default.
• W2548653394 hasConceptScore W2548653394C167734588 @default.
• W2548653394 hasConceptScore W2548653394C17991360 @default.
• W2548653394 hasConceptScore W2548653394C202751555 @default.
• W2548653394 hasConceptScore W2548653394C203014093 @default.
• W2548653394 hasConceptScore W2548653394C207001950 @default.
• W2548653394 hasConceptScore W2548653394C2776992346 @default.
• W2548653394 hasConceptScore W2548653394C2777025900 @default.
• W2548653394 hasConceptScore W2548653394C40767141 @default.
• W2548653394 hasConceptScore W2548653394C55493867 @default.
• W2548653394 hasConceptScore W2548653394C71924100 @default.
• W2548653394 hasConceptScore W2548653394C86803240 @default.
• W2548653394 hasConceptScore W2548653394C98274493 @default.
• W2548653394 hasLocation W25486533941 @default.
• W2548653394 hasLocation W25486533942 @default.
• W2548653394 hasLocation W25486533943 @default.
• W2548653394 hasOpenAccess W2548653394 @default.
• W2548653394 hasPrimaryLocation W25486533941 @default.
• W2548653394 hasRelatedWork W2016174696 @default.
• W2548653394 hasRelatedWork W2022662542 @default.
• W2548653394 hasRelatedWork W2067298762 @default.
• W2548653394 hasRelatedWork W2223453462 @default.
• W2548653394 hasRelatedWork W2349474211 @default.
• W2548653394 hasRelatedWork W2357876326 @default.
• W2548653394 hasRelatedWork W2360943677 @default.
• W2548653394 hasRelatedWork W2365309425 @default.

• next