FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2549387534> ?p ?o ?g. }

• W2549387534 endingPage "e0166621" @default.
• W2549387534 startingPage "e0166621" @default.
• W2549387534 abstract "Staphylococcus aureus is a leading cause of bovine intramammary infections (IMIs) that can evolve into difficult-to-treat chronic mastitis. To date, no vaccine formulation has shown high protective efficacy against S. aureus IMI, partly because this bacterium can efficiently evade the immune system. For instance, S. aureus small colony variants (SCVs) have intracellular abilities and can persist without producing invasive infections. As a first step towards the development of a live vaccine, this study describes the elaboration of a novel attenuated mutant of S. aureus taking advantage of the SCV phenotype. A genetically stable SCV was created through the deletion of the hemB gene, impairing its ability to adapt and revert to the invasive phenotype. Further attenuation was obtained through inactivation of gene vraG (SACOL0720) which we previously showed to be important for full virulence during bovine IMIs. After infection of bovine mammary epithelial cells (MAC-T), the double mutant (ΔvraGΔhemB) was less internalized and caused less cell destruction than that seen with ΔhemB and ΔvraG, respectively. In a murine IMI model, the ΔvraGΔhemB mutant was strongly attenuated, with a reduction of viable counts of up to 5-log10 CFU/g of mammary gland when compared to the parental strain. A complete clearance of ΔvraGΔhemB from glands was observed whereas mortality rapidly (48h) occurred with the wild-type strain. Immunization of mice using subcutaneous injections of live ΔvraGΔhemB raised a strong immune response as judged by the high total IgG titers measured against bacterial cell extracts and by the high IgG2a/IgG1 ratio observed against the IsdH protein. Also, ΔvraGΔhemB had sufficient common features with bovine mastitis strains so that the antibody response also strongly recognized strains from a variety of mastitis associated spa types. This double mutant could serve as a live-attenuated component in vaccines to improve cell-mediated immune responses against S. aureus IMIs." @default.
• W2549387534 created "2016-11-30" @default.
• W2549387534 creator A5001370851 @default.
• W2549387534 creator A5017332929 @default.
• W2549387534 creator A5035395153 @default.
• W2549387534 creator A5049253837 @default.
• W2549387534 creator A5065856436 @default.
• W2549387534 creator A5072437286 @default.
• W2549387534 date "2016-11-17" @default.
• W2549387534 modified "2023-09-23" @default.
• W2549387534 title "Characterization of a vraG Mutant in a Genetically Stable Staphylococcus aureus Small-Colony Variant and Preliminary Assessment for Use as a Live-Attenuated Vaccine against Intrammamary Infections" @default.
• W2549387534 cites W1603054792 @default.
• W2549387534 cites W1868786445 @default.
• W2549387534 cites W1901003958 @default.
• W2549387534 cites W1964205028 @default.
• W2549387534 cites W1966479702 @default.
• W2549387534 cites W1968696592 @default.
• W2549387534 cites W1969871580 @default.
• W2549387534 cites W1978087809 @default.
• W2549387534 cites W1978934797 @default.
• W2549387534 cites W1990046762 @default.
• W2549387534 cites W1991323622 @default.
• W2549387534 cites W2019760802 @default.
• W2549387534 cites W2024064751 @default.
• W2549387534 cites W2025586857 @default.
• W2549387534 cites W2026220836 @default.
• W2549387534 cites W2026663076 @default.
• W2549387534 cites W2027373007 @default.
• W2549387534 cites W2027916544 @default.
• W2549387534 cites W2028233059 @default.
• W2549387534 cites W2031189675 @default.
• W2549387534 cites W2033344929 @default.
• W2549387534 cites W2036602429 @default.
• W2549387534 cites W2042766797 @default.
• W2549387534 cites W2042984912 @default.
• W2549387534 cites W2044261540 @default.
• W2549387534 cites W2044449326 @default.
• W2549387534 cites W2046703464 @default.
• W2549387534 cites W2048425837 @default.
• W2549387534 cites W2048787993 @default.
• W2549387534 cites W2051049929 @default.
• W2549387534 cites W2051426549 @default.
• W2549387534 cites W2053524102 @default.
• W2549387534 cites W2055530572 @default.
• W2549387534 cites W2057838384 @default.
• W2549387534 cites W2067523074 @default.
• W2549387534 cites W2072572024 @default.
• W2549387534 cites W2073299375 @default.
• W2549387534 cites W2073444517 @default.
• W2549387534 cites W2080269187 @default.
• W2549387534 cites W2086731907 @default.
• W2549387534 cites W2088960594 @default.
• W2549387534 cites W2101116633 @default.
• W2549387534 cites W2105614444 @default.
• W2549387534 cites W2109132453 @default.
• W2549387534 cites W2110961750 @default.
• W2549387534 cites W2111157195 @default.
• W2549387534 cites W2111326608 @default.
• W2549387534 cites W2114910711 @default.
• W2549387534 cites W2116676275 @default.
• W2549387534 cites W2117161060 @default.
• W2549387534 cites W2117854603 @default.
• W2549387534 cites W2121050329 @default.
• W2549387534 cites W2122803099 @default.
• W2549387534 cites W2124908338 @default.
• W2549387534 cites W2134765452 @default.
• W2549387534 cites W2138731773 @default.
• W2549387534 cites W2143491155 @default.
• W2549387534 cites W2146327400 @default.
• W2549387534 cites W2147778691 @default.
• W2549387534 cites W2147988684 @default.
• W2549387534 cites W2149899666 @default.
• W2549387534 cites W2151150074 @default.
• W2549387534 cites W2154661271 @default.
• W2549387534 cites W2156750860 @default.
• W2549387534 cites W2157468333 @default.
• W2549387534 cites W2160418143 @default.
• W2549387534 cites W2162549023 @default.
• W2549387534 cites W2163919171 @default.
• W2549387534 cites W2166144613 @default.
• W2549387534 cites W2166180850 @default.
• W2549387534 cites W2171385213 @default.
• W2549387534 cites W2205758324 @default.
• W2549387534 cites W2256679284 @default.
• W2549387534 doi "https://doi.org/10.1371/journal.pone.0166621" @default.
• W2549387534 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5113970" @default.
• W2549387534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27855187" @default.
• W2549387534 hasPublicationYear "2016" @default.
• W2549387534 type Work @default.
• W2549387534 sameAs 2549387534 @default.
• W2549387534 citedByCount "17" @default.
• W2549387534 countsByYear W25493875342018 @default.
• W2549387534 countsByYear W25493875342019 @default.
• W2549387534 countsByYear W25493875342020 @default.
• W2549387534 countsByYear W25493875342021 @default.
• W2549387534 countsByYear W25493875342022 @default.
• W2549387534 countsByYear W25493875342023 @default.
• W2549387534 crossrefType "journal-article" @default.

• next