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- W254953800 abstract "This chapter discusses the biosynthetic radio-labeling of cellular and secreted proteins of mononuclear phagocytes. A complete description of the phenotype of mononuclear phagocytes involves defining all the biochemical and functional properties of the cells. One approach is to examine the pattern of total transcribable mRNA present in a macrophage at any given point in its history. This can be achieved either by translating isolated mRNA in cell-free systems, a process unsuitable for the examination of many samples, or by studying the translation of mRNA into proteins using the machinery of a live cell. If radio-labeled amino acids are present during translation in live cells, the resulting biosynthesized proteins can be analyzed by polyacrylamide gel electrophoresis to give detailed fingerprints of specific macrophage phenotypes. In conjunction with other methods, such as specific immunoprecipitation of labeled proteins, it is possible to examine detail changes in the properties of macrophages that may not be seen with a single assay such as receptor binding or quantification of a secreted proteolytic enzyme." @default.
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- W254953800 date "1981-01-01" @default.
- W254953800 modified "2023-09-25" @default.
- W254953800 title "BIOSYNTHETIC RADIOLABELING OF CELLULAR AND SECRETED PROTEINS OF MONONUCLEAR PHAGOCYTES" @default.
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- W254953800 doi "https://doi.org/10.1016/b978-0-12-044220-1.50086-6" @default.
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