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• W2549548257 endingPage "29" @default.
• W2549548257 startingPage "18" @default.
• W2549548257 abstract "In order to clarify the suitability of the lateral line of zebrafish (Danio rerio) embryos as a model for the screening of ototoxic (neurotoxic) effects, existing neuromast assays were adapted, improved and validated with a series of chemicals known or unknown for their ototoxic potential (caffeine copper sulfate, dichlorvos, 2.4-dinitrotoluene, neomycin, 4-nonylphenol, perfluorooctanesulfonic acid). Present methods were improved by (1) the introduction of a 4-step scoring system, (2) the selection of neuromasts from both the anterior and posterior lateral line systems, (3) a combined DASPEI/DAPI staining applied after both a continuous and pulse exposure scenario, and (4) an additional screening for nuclear fragmentation. Acute toxicities of the model substances were determined by means of the fish embryo test as specified in OECD TG 236, and EC10 concentrations were used as the highest test concentration in the neuromast assay. The enhanced neuromast assay identified known ototoxic substances such as neomycin and copper sulfate as ototoxic at sensitivities similar to those of established methods, with pulse exposure leading to stronger effects than continuous exposure. Except for caffeine, all substances tested (dichlorvos, 2.4-dinitrotoluene, 4-nonylphenol, perfluorooctanesulfonic acid) produced significant toxic effects in neuromasts at EC10 concentrations. Depending on the test substances and their location along the lateral line, specific neuromasts differed in sensitivity. Generally, neuromasts proved more sensitive in the pulse exposure scenario. Whereas for neomycin and copper sulfate neuromasts located along the anterior lateral line were more sensitive, posterior lateral line neuromasts proved more sensitive for the other test substances. Nuclear fragmentation could not only be associated with all test substances, but, albeit at lower frequencies, also with negative controls, and could, therefore, not be assigned specifically to chemical damage. The study thus documented that for a comprehensive evaluation of lateral line damage both neuromasts from the anterior and the posterior lateral line have to be considered. Given the apparently rapid regeneration of hair cells, pulse exposure seems more appropriate for the identification of lateral line neurotoxicity than continuous exposure." @default.
• W2549548257 created "2016-11-30" @default.
• W2549548257 creator A5028256622 @default.
• W2549548257 creator A5042450452 @default.
• W2549548257 creator A5053037157 @default.
• W2549548257 date "2017-03-01" @default.
• W2549548257 modified "2023-09-24" @default.
• W2549548257 title "An optimized method to assess ototoxic effects in the lateral line of zebrafish (Danio rerio) embryos" @default.
• W2549548257 cites W1493367564 @default.
• W2549548257 cites W1695724483 @default.
• W2549548257 cites W1874253610 @default.
• W2549548257 cites W1963506121 @default.
• W2549548257 cites W1964137615 @default.
• W2549548257 cites W1968495751 @default.
• W2549548257 cites W1971305333 @default.
• W2549548257 cites W1972541502 @default.
• W2549548257 cites W1972637360 @default.
• W2549548257 cites W1974714134 @default.
• W2549548257 cites W1977690514 @default.
• W2549548257 cites W1981716282 @default.
• W2549548257 cites W1982119955 @default.
• W2549548257 cites W1984109965 @default.
• W2549548257 cites W1985106232 @default.
• W2549548257 cites W1985134047 @default.
• W2549548257 cites W1990199992 @default.
• W2549548257 cites W1996649210 @default.
• W2549548257 cites W1997495771 @default.
• W2549548257 cites W1999420091 @default.
• W2549548257 cites W2004281170 @default.
• W2549548257 cites W2005958745 @default.
• W2549548257 cites W2006179033 @default.
• W2549548257 cites W2010007119 @default.
• W2549548257 cites W2010020384 @default.
• W2549548257 cites W2011467689 @default.
• W2549548257 cites W2011930646 @default.
• W2549548257 cites W2012321949 @default.
• W2549548257 cites W2013020096 @default.
• W2549548257 cites W2013337492 @default.
• W2549548257 cites W2014235257 @default.
• W2549548257 cites W2016375494 @default.
• W2549548257 cites W2016846420 @default.
• W2549548257 cites W2020009339 @default.
• W2549548257 cites W2020882905 @default.
• W2549548257 cites W2021697970 @default.
• W2549548257 cites W2026302267 @default.
• W2549548257 cites W2026805828 @default.
• W2549548257 cites W2030382945 @default.
• W2549548257 cites W2032329802 @default.
• W2549548257 cites W2033827034 @default.
• W2549548257 cites W2040558296 @default.
• W2549548257 cites W2043499474 @default.
• W2549548257 cites W2047492819 @default.
• W2549548257 cites W2047735796 @default.
• W2549548257 cites W2054016792 @default.
• W2549548257 cites W2054378694 @default.
• W2549548257 cites W2057311667 @default.
• W2549548257 cites W2057995143 @default.
• W2549548257 cites W2060811345 @default.
• W2549548257 cites W2060948257 @default.
• W2549548257 cites W2064507515 @default.
• W2549548257 cites W2066308752 @default.
• W2549548257 cites W2067371498 @default.
• W2549548257 cites W2067388136 @default.
• W2549548257 cites W2069158023 @default.
• W2549548257 cites W2072316722 @default.
• W2549548257 cites W2074053057 @default.
• W2549548257 cites W2074322477 @default.
• W2549548257 cites W2074454978 @default.
• W2549548257 cites W2075047454 @default.
• W2549548257 cites W2077942428 @default.
• W2549548257 cites W2078664739 @default.
• W2549548257 cites W2079167165 @default.
• W2549548257 cites W2082857620 @default.
• W2549548257 cites W2083329120 @default.
• W2549548257 cites W2085283725 @default.
• W2549548257 cites W2085992814 @default.
• W2549548257 cites W2087984711 @default.
• W2549548257 cites W2088249051 @default.
• W2549548257 cites W2088421193 @default.
• W2549548257 cites W2088708637 @default.
• W2549548257 cites W2090114628 @default.
• W2549548257 cites W2091508032 @default.
• W2549548257 cites W2091559596 @default.
• W2549548257 cites W2092472738 @default.
• W2549548257 cites W2092888258 @default.
• W2549548257 cites W2093168078 @default.
• W2549548257 cites W2094060256 @default.
• W2549548257 cites W2096482948 @default.
• W2549548257 cites W2107708916 @default.
• W2549548257 cites W2116929458 @default.
• W2549548257 cites W2120506049 @default.
• W2549548257 cites W2125349610 @default.
• W2549548257 cites W2129090736 @default.
• W2549548257 cites W2133358624 @default.
• W2549548257 cites W2134883557 @default.
• W2549548257 cites W2137701107 @default.
• W2549548257 cites W2140858853 @default.
• W2549548257 cites W2144469901 @default.

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