FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2549586825> ?p ?o ?g. }

• W2549586825 endingPage "154" @default.
• W2549586825 startingPage "143" @default.
• W2549586825 abstract "Axial spondyloarthritis (axSpA), which encompasses ankylosing spondylitis, is a complex genetic disease. Aberrant bone formation is a key feature of pathogenesis that can lead to ankylosis of the spine. Our objective is to determine, whether genes whose variants confer susceptibility to AS are expressed in bone progenitors like mesenchymal stem cells (MSCs). Since MSCs from bone marrow is difficult to obtain, we first examined, whether MSCs can be derived from induced pluripotent stem cells (iPSCs). Dermal fibroblasts of two axSpA patients and one healthy control were reprogrammed into iPSCs using a Sendai virus vector encoding pluripotency genes. Pluripotency of iPSCs was examined by embryoid body formation and by testing for stem cell specific gene and protein expression using RT-PCR and immuno fluorescence. iPSCs were differentiated into MSCs by a TGFß inhibitor. MSCs were characterized by flow cytometry using lineage specific antibodies and by their capacity to develop into chondrocytes, adipocytes, and osteoblasts in lineage-specific medium. RNA-seq was applied to determine genome-wide gene expression patterns in MSCs, iPSCs, and blood. We show for the first time, that expression levels of several AS susceptibility genes (EDIL3, ANO6, HAPLN1, ANTXR2) involved in bone formation are significantly elevated in MSCs (2–15-fold; p ≤ 0.05) compared to blood or iPSCs and demonstrate that iPSC-derived MSCs can be differentiated into osteoblasts, chondrocytes, and adipocytes. We conclude, MSCs generated from patient fibroblast-derived iPSC lines are useful tools for studying functional genomics of risk genes associated with bone formation in AS pathogenesis." @default.
• W2549586825 created "2016-11-30" @default.
• W2549586825 creator A5002704860 @default.
• W2549586825 creator A5022999797 @default.
• W2549586825 creator A5040260909 @default.
• W2549586825 creator A5040534586 @default.
• W2549586825 creator A5043926752 @default.
• W2549586825 creator A5045276116 @default.
• W2549586825 creator A5076557814 @default.
• W2549586825 creator A5078434832 @default.
• W2549586825 creator A5079918193 @default.
• W2549586825 date "2016-11-18" @default.
• W2549586825 modified "2023-10-02" @default.
• W2549586825 title "Generation and differentiation of induced pluripotent stem cells reveal ankylosing spondylitis risk gene expression in bone progenitors" @default.
• W2549586825 cites W1496723053 @default.
• W2549586825 cites W1780842662 @default.
• W2549586825 cites W1906201952 @default.
• W2549586825 cites W1961692905 @default.
• W2549586825 cites W1981309002 @default.
• W2549586825 cites W1983251816 @default.
• W2549586825 cites W2015905253 @default.
• W2549586825 cites W2027454708 @default.
• W2549586825 cites W2048020246 @default.
• W2549586825 cites W2052328320 @default.
• W2549586825 cites W2052917232 @default.
• W2549586825 cites W2053988054 @default.
• W2549586825 cites W2056365441 @default.
• W2549586825 cites W2062019717 @default.
• W2549586825 cites W2074649793 @default.
• W2549586825 cites W2077062756 @default.
• W2549586825 cites W2078635052 @default.
• W2549586825 cites W2084201375 @default.
• W2549586825 cites W2097339629 @default.
• W2549586825 cites W2097896494 @default.
• W2549586825 cites W2106025753 @default.
• W2549586825 cites W2107909189 @default.
• W2549586825 cites W2108571057 @default.
• W2549586825 cites W2110228129 @default.
• W2549586825 cites W2112409097 @default.
• W2549586825 cites W2115942040 @default.
• W2549586825 cites W2117004211 @default.
• W2549586825 cites W2117800935 @default.
• W2549586825 cites W2121782932 @default.
• W2549586825 cites W2123758371 @default.
• W2549586825 cites W2125987139 @default.
• W2549586825 cites W2136087193 @default.
• W2549586825 cites W2138977668 @default.
• W2549586825 cites W2142046859 @default.
• W2549586825 cites W2155062057 @default.
• W2549586825 cites W2155615155 @default.
• W2549586825 cites W2156605473 @default.
• W2549586825 cites W2163329733 @default.
• W2549586825 cites W2167754556 @default.
• W2549586825 cites W2169639299 @default.
• W2549586825 cites W2274426412 @default.
• W2549586825 cites W2278932593 @default.
• W2549586825 cites W2474510828 @default.
• W2549586825 doi "https://doi.org/10.1007/s10067-016-3469-5" @default.
• W2549586825 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5216109" @default.
• W2549586825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27864696" @default.
• W2549586825 hasPublicationYear "2016" @default.
• W2549586825 type Work @default.
• W2549586825 sameAs 2549586825 @default.
• W2549586825 citedByCount "15" @default.
• W2549586825 countsByYear W25495868252017 @default.
• W2549586825 countsByYear W25495868252018 @default.
• W2549586825 countsByYear W25495868252019 @default.
• W2549586825 countsByYear W25495868252020 @default.
• W2549586825 countsByYear W25495868252021 @default.
• W2549586825 countsByYear W25495868252022 @default.
• W2549586825 countsByYear W25495868252023 @default.
• W2549586825 crossrefType "journal-article" @default.
• W2549586825 hasAuthorship W2549586825A5002704860 @default.
• W2549586825 hasAuthorship W2549586825A5022999797 @default.
• W2549586825 hasAuthorship W2549586825A5040260909 @default.
• W2549586825 hasAuthorship W2549586825A5040534586 @default.
• W2549586825 hasAuthorship W2549586825A5043926752 @default.
• W2549586825 hasAuthorship W2549586825A5045276116 @default.
• W2549586825 hasAuthorship W2549586825A5076557814 @default.
• W2549586825 hasAuthorship W2549586825A5078434832 @default.
• W2549586825 hasAuthorship W2549586825A5079918193 @default.
• W2549586825 hasBestOaLocation W25495868252 @default.
• W2549586825 hasConcept C104317684 @default.
• W2549586825 hasConcept C107459253 @default.
• W2549586825 hasConcept C13685319 @default.
• W2549586825 hasConcept C145103041 @default.
• W2549586825 hasConcept C148738053 @default.
• W2549586825 hasConcept C198826908 @default.
• W2549586825 hasConcept C201750760 @default.
• W2549586825 hasConcept C203014093 @default.
• W2549586825 hasConcept C20875687 @default.
• W2549586825 hasConcept C2779310769 @default.
• W2549586825 hasConcept C28328180 @default.
• W2549586825 hasConcept C502942594 @default.
• W2549586825 hasConcept C54355233 @default.
• W2549586825 hasConcept C71924100 @default.
• W2549586825 hasConcept C86803240 @default.
• W2549586825 hasConcept C95444343 @default.

• next