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- W2549616524 abstract "TheMcrAandMcrB(modified cytosine restriction) systems ofE.coli interfere withincoming DNA containing methylcytosine. DNA frommanyorganisms, including allmammalian andplant DNA, isexpected tobesensitive, andthis could interfere withcloning experiments. TheMcrAandBphenotypes ofafewstrains havebeenreported previously (1-4). TheMcrphenotypes of94strains, primarily derived fromE.coli K12,aretabulated here. Webriefly review someevidence suggesting thatMcrB restriction ofmouse-modified DNA doesoccurinvivoanddoesinfact interfere withcloning ofspecific mousesequences." @default.
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- W2549616524 date "1987-01-01" @default.
- W2549616524 modified "2023-10-13" @default.
- W2549616524 title "McrAandMcrBrestriction phenotypes ofsomeE.coli strains andimplications forgenecloning" @default.
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