FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2549677965> ?p ?o ?g. }

• W2549677965 endingPage "12326" @default.
• W2549677965 startingPage "12321" @default.
• W2549677965 abstract "Previously, an electron spin echo envelope modulation (ESEEM) spectroscopic approach was established to probe the local secondary structure of membrane proteins and peptides utilizing site-directed spin-labeling (SDSL). In this method, the side chain of one amino acid residue is selectively 2H-labeled and a nitroxide spin label is strategically placed 1, 2, 3, or 4 amino acids away from the 2H-labeled amino acid (denoted as i ± 1 to i ± 4, i represents the 2H-labeled amino acid). ESEEM can detect the dipolar coupling between the nitroxide spin label and 2H atoms on the amino acid side chain. Due to the periodicity of different secondary structures, different ESEEM patterns can be revealed to probe the structure. For an α-helical structural component, a 2H ESEEM signal can be detected for i ± 3 and i ± 4 samples, but not for i ± 1 or i ± 2 samples. Several 2H-labeled hydrophobic amino acids have been demonstrated in model system that can be utilized to identify local secondary structures via this ESEEM approach in an extremely efficient fashion. In this study, the ESEEM approach was used to investigate the rod 2B region of the full-length intermediate filament protein human vimentin. Consistent with previous EPR and X-ray crystallography results, our ESEEM results indicated helical structural components within this region. Thus, this ESEEM approach is able to identify α-helical structural components despite the coiled-coil nature of the vimentin structure. The data show that the human vimentin rod 2B adapted a typical α-helical structure around residue Leu309. This result is consistent with the X-ray data from fragmented protein segments and continuous wave EPR data on the full-length vimentin. Finally, the ESEEM data suggested that a local secondary structure slightly different from a typical α-helix was adopted around residue 340." @default.
• W2549677965 created "2016-11-30" @default.
• W2549677965 creator A5000389536 @default.
• W2549677965 creator A5000609624 @default.
• W2549677965 creator A5057420306 @default.
• W2549677965 creator A5060490393 @default.
• W2549677965 creator A5068710042 @default.
• W2549677965 creator A5081009879 @default.
• W2549677965 date "2016-11-28" @default.
• W2549677965 modified "2023-09-26" @default.
• W2549677965 title "Probing the Local Secondary Structure of Human Vimentin with Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy" @default.
• W2549677965 cites W1482444677 @default.
• W2549677965 cites W1973010837 @default.
• W2549677965 cites W1977499446 @default.
• W2549677965 cites W1978023710 @default.
• W2549677965 cites W1983007565 @default.
• W2549677965 cites W1986517464 @default.
• W2549677965 cites W1995278329 @default.
• W2549677965 cites W1997820347 @default.
• W2549677965 cites W2001963734 @default.
• W2549677965 cites W2016766380 @default.
• W2549677965 cites W2019701338 @default.
• W2549677965 cites W2025346903 @default.
• W2549677965 cites W2033452569 @default.
• W2549677965 cites W2034292992 @default.
• W2549677965 cites W2037970878 @default.
• W2549677965 cites W2054192060 @default.
• W2549677965 cites W2076328566 @default.
• W2549677965 cites W2079608656 @default.
• W2549677965 cites W2103731288 @default.
• W2549677965 cites W2105280782 @default.
• W2549677965 cites W2105502557 @default.
• W2549677965 cites W2124026286 @default.
• W2549677965 cites W2142353117 @default.
• W2549677965 cites W2151457078 @default.
• W2549677965 cites W2151780134 @default.
• W2549677965 cites W2155720843 @default.
• W2549677965 cites W2165887549 @default.
• W2549677965 cites W2337494987 @default.
• W2549677965 cites W3342766 @default.
• W2549677965 doi "https://doi.org/10.1021/acs.jpcb.6b10054" @default.
• W2549677965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27934222" @default.
• W2549677965 hasPublicationYear "2016" @default.
• W2549677965 type Work @default.
• W2549677965 sameAs 2549677965 @default.
• W2549677965 citedByCount "13" @default.
• W2549677965 countsByYear W25496779652017 @default.
• W2549677965 countsByYear W25496779652018 @default.
• W2549677965 countsByYear W25496779652019 @default.
• W2549677965 countsByYear W25496779652020 @default.
• W2549677965 countsByYear W25496779652021 @default.
• W2549677965 countsByYear W25496779652022 @default.
• W2549677965 crossrefType "journal-article" @default.
• W2549677965 hasAuthorship W2549677965A5000389536 @default.
• W2549677965 hasAuthorship W2549677965A5000609624 @default.
• W2549677965 hasAuthorship W2549677965A5057420306 @default.
• W2549677965 hasAuthorship W2549677965A5060490393 @default.
• W2549677965 hasAuthorship W2549677965A5068710042 @default.
• W2549677965 hasAuthorship W2549677965A5081009879 @default.
• W2549677965 hasConcept C104317684 @default.
• W2549677965 hasConcept C111998727 @default.
• W2549677965 hasConcept C121332964 @default.
• W2549677965 hasConcept C14228908 @default.
• W2549677965 hasConcept C147447768 @default.
• W2549677965 hasConcept C15920480 @default.
• W2549677965 hasConcept C167625842 @default.
• W2549677965 hasConcept C169912494 @default.
• W2549677965 hasConcept C177462420 @default.
• W2549677965 hasConcept C178790620 @default.
• W2549677965 hasConcept C185592680 @default.
• W2549677965 hasConcept C187961010 @default.
• W2549677965 hasConcept C203014093 @default.
• W2549677965 hasConcept C204232928 @default.
• W2549677965 hasConcept C2994376932 @default.
• W2549677965 hasConcept C46141821 @default.
• W2549677965 hasConcept C47701112 @default.
• W2549677965 hasConcept C515207424 @default.
• W2549677965 hasConcept C521977710 @default.
• W2549677965 hasConcept C55493867 @default.
• W2549677965 hasConcept C55988834 @default.
• W2549677965 hasConcept C62614982 @default.
• W2549677965 hasConcept C79123411 @default.
• W2549677965 hasConcept C8010536 @default.
• W2549677965 hasConcept C86803240 @default.
• W2549677965 hasConceptScore W2549677965C104317684 @default.
• W2549677965 hasConceptScore W2549677965C111998727 @default.
• W2549677965 hasConceptScore W2549677965C121332964 @default.
• W2549677965 hasConceptScore W2549677965C14228908 @default.
• W2549677965 hasConceptScore W2549677965C147447768 @default.
• W2549677965 hasConceptScore W2549677965C15920480 @default.
• W2549677965 hasConceptScore W2549677965C167625842 @default.
• W2549677965 hasConceptScore W2549677965C169912494 @default.
• W2549677965 hasConceptScore W2549677965C177462420 @default.
• W2549677965 hasConceptScore W2549677965C178790620 @default.
• W2549677965 hasConceptScore W2549677965C185592680 @default.
• W2549677965 hasConceptScore W2549677965C187961010 @default.
• W2549677965 hasConceptScore W2549677965C203014093 @default.
• W2549677965 hasConceptScore W2549677965C204232928 @default.

• next