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- W2549830781 abstract "Characterization of viral DNA methylation patterns is essential to understand its function in viral pathogenesis. Bisulfite modification, followed by polymerase chain reaction (PCR) and sequencing is the most effective method for the high resolution methylation mapping of viral genomes. Since the bisulfite modification and PCR steps are the most critical ones, an optimized protocol for these two steps is presented, with special attention to potential pitfalls." @default.
- W2549830781 created "2016-11-30" @default.
- W2549830781 creator A5029450322 @default.
- W2549830781 date "2016-11-22" @default.
- W2549830781 modified "2023-10-02" @default.
- W2549830781 title "Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps" @default.
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- W2549830781 doi "https://doi.org/10.1007/978-1-4939-6655-4_15" @default.
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