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• W2550173864 abstract "The pathogenesis of metastasis of colon cancer (Cca) is to be further investigated. The dysfunction of apoptotic mechanism plays a role in the cancer cell over growth. This study tests a hypothesis by which intestinal bacterium-derived cyp27a1 prevents apoptosis in colon cancer cells. In this study, the levels of cyp27a1 in human stool samples were assessed by enzyme-linked immunosorbent assay. The apoptosis of Cca cells was observed by flow cytometry. The expression of cyp27a1 was assessed by real time RT-PCR and Western blotting. We observed higher levels of cyp27a1 in the stool samples of Cca patients than that from healthy subjects. Cca colon epithelial biopsy contained high levels of cyp27a1 protein, but not the cyp27a1 mRNA. Cyp27a1 prevented Cca cell apoptosis induced by vitamin D3. In conclusion, intestinal bacterium-derived cyp27a1 facilitates Cca survival by inhibiting Cca cell apoptosis." @default.
• W2550173864 created "2016-11-30" @default.
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• W2550173864 date "2016-01-01" @default.
• W2550173864 modified "2023-10-16" @default.
• W2550173864 title "Intestinal bacterium-derived cyp27a1 prevents colon cancer cell apoptosis." @default.
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• W2550173864 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5095336" @default.
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