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• W2550240464 abstract "As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at -20 °C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels. PMA-PCR assay for VBNC detection was also validated. Therefore, these results suggest that VBNC E. coli O157 strains may represent a strong threat to public health and food safety." @default.
• W2550240464 created "2016-11-30" @default.
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• W2550240464 date "2017-04-01" @default.
• W2550240464 modified "2023-10-09" @default.
• W2550240464 title "Viable but non-culturable state and toxin gene expression of enterohemorrhagic Escherichia coli O157 under cryopreservation" @default.
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• W2550240464 doi "https://doi.org/10.1016/j.resmic.2016.11.002" @default.
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