Matches in SemOpenAlex for { <https://semopenalex.org/work/W2550242024> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2550242024 abstract "Following the cloning and expression of tobacco (Nicotiana tabacum) phytaspase gene in Escherichia coli BL21, the recombinant protease was purified by affinity chromatography for further characterization. Circular dichroism (CD) spectroscopy and in silico analysis revealed structural similarities of recombinant phytaspase with other plant serine-proteases. Molecular docking studies showed favourable binding of synthetic peptide substrate for caspase 8 (Ac-VETD-AMC) to the reactive pocket of recombinant phytaspase indicating its potential in assessing functional activity of recombinant phytaspase. In silico findings were supported by caspase 8-like activity of purified phytaspase demonstrated in vitro. The Michaelis constant (KM) and specificity constant (kcat/KM) of phytaspase for hydrolyzing Ac-VETD-AMC were found to be 1.587μM and 4.67×103M-1min-1, respectively. Transient expression of phytaspase in lung epithelial adenocarcinoma cells (A549) resulted in reduced IC50 value of doxorubicin. This is the first report of functional expression of mature phytaspase in bacterial system as well as its transfection to sensitize A549 cells at lower doxorubicin concentration." @default.
- W2550242024 created "2016-11-30" @default.
- W2550242024 creator A5014865113 @default.
- W2550242024 creator A5038797225 @default.
- W2550242024 creator A5055795146 @default.
- W2550242024 creator A5062151143 @default.
- W2550242024 date "2017-02-01" @default.
- W2550242024 modified "2023-09-22" @default.
- W2550242024 title "Heterologous expression and functional characterization of phytaspase, a caspase-like plant protease" @default.
- W2550242024 cites W1967448664 @default.
- W2550242024 cites W2005715606 @default.
- W2550242024 cites W2030429042 @default.
- W2550242024 cites W2059994793 @default.
- W2550242024 cites W2060180453 @default.
- W2550242024 cites W2068140007 @default.
- W2550242024 cites W2078010779 @default.
- W2550242024 cites W2085420192 @default.
- W2550242024 cites W2090951206 @default.
- W2550242024 cites W2140256954 @default.
- W2550242024 cites W2154437470 @default.
- W2550242024 cites W2157952797 @default.
- W2550242024 cites W2158070520 @default.
- W2550242024 cites W2160334612 @default.
- W2550242024 cites W2194218235 @default.
- W2550242024 cites W2258471378 @default.
- W2550242024 cites W2319173743 @default.
- W2550242024 cites W2320389941 @default.
- W2550242024 cites W86994470 @default.
- W2550242024 doi "https://doi.org/10.1016/j.ijbiomac.2016.11.058" @default.
- W2550242024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27867055" @default.
- W2550242024 hasPublicationYear "2017" @default.
- W2550242024 type Work @default.
- W2550242024 sameAs 2550242024 @default.
- W2550242024 citedByCount "3" @default.
- W2550242024 countsByYear W25502420242017 @default.
- W2550242024 countsByYear W25502420242023 @default.
- W2550242024 crossrefType "journal-article" @default.
- W2550242024 hasAuthorship W2550242024A5014865113 @default.
- W2550242024 hasAuthorship W2550242024A5038797225 @default.
- W2550242024 hasAuthorship W2550242024A5055795146 @default.
- W2550242024 hasAuthorship W2550242024A5062151143 @default.
- W2550242024 hasConcept C104317684 @default.
- W2550242024 hasConcept C108305142 @default.
- W2550242024 hasConcept C133571119 @default.
- W2550242024 hasConcept C153911025 @default.
- W2550242024 hasConcept C181199279 @default.
- W2550242024 hasConcept C182220744 @default.
- W2550242024 hasConcept C185592680 @default.
- W2550242024 hasConcept C202751555 @default.
- W2550242024 hasConcept C2776714187 @default.
- W2550242024 hasConcept C2781205006 @default.
- W2550242024 hasConcept C40767141 @default.
- W2550242024 hasConcept C547475151 @default.
- W2550242024 hasConcept C55493867 @default.
- W2550242024 hasConcept C81729549 @default.
- W2550242024 hasConcept C86803240 @default.
- W2550242024 hasConceptScore W2550242024C104317684 @default.
- W2550242024 hasConceptScore W2550242024C108305142 @default.
- W2550242024 hasConceptScore W2550242024C133571119 @default.
- W2550242024 hasConceptScore W2550242024C153911025 @default.
- W2550242024 hasConceptScore W2550242024C181199279 @default.
- W2550242024 hasConceptScore W2550242024C182220744 @default.
- W2550242024 hasConceptScore W2550242024C185592680 @default.
- W2550242024 hasConceptScore W2550242024C202751555 @default.
- W2550242024 hasConceptScore W2550242024C2776714187 @default.
- W2550242024 hasConceptScore W2550242024C2781205006 @default.
- W2550242024 hasConceptScore W2550242024C40767141 @default.
- W2550242024 hasConceptScore W2550242024C547475151 @default.
- W2550242024 hasConceptScore W2550242024C55493867 @default.
- W2550242024 hasConceptScore W2550242024C81729549 @default.
- W2550242024 hasConceptScore W2550242024C86803240 @default.
- W2550242024 hasFunder F4320326716 @default.
- W2550242024 hasLocation W25502420241 @default.
- W2550242024 hasLocation W25502420242 @default.
- W2550242024 hasOpenAccess W2550242024 @default.
- W2550242024 hasPrimaryLocation W25502420241 @default.
- W2550242024 hasRelatedWork W1531475302 @default.
- W2550242024 hasRelatedWork W1969505841 @default.
- W2550242024 hasRelatedWork W1970861952 @default.
- W2550242024 hasRelatedWork W1990598600 @default.
- W2550242024 hasRelatedWork W1994532826 @default.
- W2550242024 hasRelatedWork W1998610805 @default.
- W2550242024 hasRelatedWork W2027803844 @default.
- W2550242024 hasRelatedWork W2035017225 @default.
- W2550242024 hasRelatedWork W2038527714 @default.
- W2550242024 hasRelatedWork W2039545449 @default.
- W2550242024 hasRelatedWork W2054318435 @default.
- W2550242024 hasRelatedWork W2071027165 @default.
- W2550242024 hasRelatedWork W2074931244 @default.
- W2550242024 hasRelatedWork W2075584954 @default.
- W2550242024 hasRelatedWork W2077560184 @default.
- W2550242024 hasRelatedWork W2409653369 @default.
- W2550242024 hasRelatedWork W2423676132 @default.
- W2550242024 hasRelatedWork W2728241708 @default.
- W2550242024 hasRelatedWork W2993315506 @default.
- W2550242024 hasRelatedWork W2188295555 @default.
- W2550242024 isParatext "false" @default.
- W2550242024 isRetracted "false" @default.
- W2550242024 magId "2550242024" @default.
- W2550242024 workType "article" @default.