Matches in SemOpenAlex for { <https://semopenalex.org/work/W2550295393> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2550295393 abstract "Traditional breeding as well as genetic engineering have been utilized to improve drought and salinity tolerance of rice with the goal of increasing its productivity. Use of modern molecular biology tools for engineering stress tolerant crops is based on the expression of specific stress-related genes. So, the choice of promoter can play an important role in abiotic stress tolerance as it controls gene expression. Thus inducible expression of gene is crucial for transgenic rice research which is usually implemented by making use of inducible promoters. In this study, therefore, a stress inducible promoter RD29A from Arabidopsis was characterized in Binnatoa rice variety. For this characterization, Agrobacterium mediated transformation of Binnatoa rice was carried out using the RD29A-GUS construct. The transgene was confirmed up to T2 generation by RD29A specific primer. Southern analysis indicated single-copy integration of the promoter in the rice genome. Histochemical assay was performed in rice leaves, roots and seeds to test the inducibility of RD29A under both drought and salt stress (100 mM and 200 mM). Salt stress was applied at both seedling and reproductive stages whereas drought stress was applied only at seedling stage. Under drought stress, no expression was visible in root samples but a very prominent expression was found in leaves at day 7, 10, 13 and 16. At 100 mM salt stress, color intensity indicated gradual GUS expression increase up to 24h but a decline at 48h in both leaves and roots. At 200 mM salt stress however, expression of GUS in leaf sample was found remarkably higher at 5, 10, 24 and 48 hours. In the seedling root however, highest color was found after 5 hours of 200 mM stress. At reproductive stage stress, at both 100 and 200 mM salt exposure 6 days after flowering, GUS expression was maximal in leaves and seeds at 24 hours, but no expression was detected in roots. The time required for the toxicity of the salt to take effect, particularly in photosynthesizing leaves is about 24 hours. Therefore, maximal expression of the GUS gene at 24 hours indicates the suitability of the RD29A promoter for driving transgenes suitable in conferring salt tolerance. This promoter was also found suitable for driving transgenes in leaves under drought stress." @default.
- W2550295393 created "2016-11-30" @default.
- W2550295393 creator A5033958016 @default.
- W2550295393 creator A5076086343 @default.
- W2550295393 creator A5081888363 @default.
- W2550295393 creator A5082408982 @default.
- W2550295393 creator A5086294755 @default.
- W2550295393 date "2016-01-01" @default.
- W2550295393 modified "2023-09-27" @default.
- W2550295393 title "Cloning, characterization and analysis of the Arabidopsis RD29A promoter for its inducible expression in rice under salinity and drought stress" @default.
- W2550295393 cites W1577292085 @default.
- W2550295393 cites W2144634347 @default.
- W2550295393 hasPublicationYear "2016" @default.
- W2550295393 type Work @default.
- W2550295393 sameAs 2550295393 @default.
- W2550295393 citedByCount "0" @default.
- W2550295393 crossrefType "journal-article" @default.
- W2550295393 hasAuthorship W2550295393A5033958016 @default.
- W2550295393 hasAuthorship W2550295393A5076086343 @default.
- W2550295393 hasAuthorship W2550295393A5081888363 @default.
- W2550295393 hasAuthorship W2550295393A5082408982 @default.
- W2550295393 hasAuthorship W2550295393A5086294755 @default.
- W2550295393 hasConcept C101762097 @default.
- W2550295393 hasConcept C102230213 @default.
- W2550295393 hasConcept C104317684 @default.
- W2550295393 hasConcept C104320060 @default.
- W2550295393 hasConcept C121050878 @default.
- W2550295393 hasConcept C122172344 @default.
- W2550295393 hasConcept C143065580 @default.
- W2550295393 hasConcept C144027150 @default.
- W2550295393 hasConcept C150194340 @default.
- W2550295393 hasConcept C150903083 @default.
- W2550295393 hasConcept C177955015 @default.
- W2550295393 hasConcept C199360897 @default.
- W2550295393 hasConcept C204241405 @default.
- W2550295393 hasConcept C2776096895 @default.
- W2550295393 hasConcept C2778543611 @default.
- W2550295393 hasConcept C2779491563 @default.
- W2550295393 hasConcept C2779656422 @default.
- W2550295393 hasConcept C41008148 @default.
- W2550295393 hasConcept C54355233 @default.
- W2550295393 hasConcept C59822182 @default.
- W2550295393 hasConcept C86803240 @default.
- W2550295393 hasConcept C89519541 @default.
- W2550295393 hasConceptScore W2550295393C101762097 @default.
- W2550295393 hasConceptScore W2550295393C102230213 @default.
- W2550295393 hasConceptScore W2550295393C104317684 @default.
- W2550295393 hasConceptScore W2550295393C104320060 @default.
- W2550295393 hasConceptScore W2550295393C121050878 @default.
- W2550295393 hasConceptScore W2550295393C122172344 @default.
- W2550295393 hasConceptScore W2550295393C143065580 @default.
- W2550295393 hasConceptScore W2550295393C144027150 @default.
- W2550295393 hasConceptScore W2550295393C150194340 @default.
- W2550295393 hasConceptScore W2550295393C150903083 @default.
- W2550295393 hasConceptScore W2550295393C177955015 @default.
- W2550295393 hasConceptScore W2550295393C199360897 @default.
- W2550295393 hasConceptScore W2550295393C204241405 @default.
- W2550295393 hasConceptScore W2550295393C2776096895 @default.
- W2550295393 hasConceptScore W2550295393C2778543611 @default.
- W2550295393 hasConceptScore W2550295393C2779491563 @default.
- W2550295393 hasConceptScore W2550295393C2779656422 @default.
- W2550295393 hasConceptScore W2550295393C41008148 @default.
- W2550295393 hasConceptScore W2550295393C54355233 @default.
- W2550295393 hasConceptScore W2550295393C59822182 @default.
- W2550295393 hasConceptScore W2550295393C86803240 @default.
- W2550295393 hasConceptScore W2550295393C89519541 @default.
- W2550295393 hasLocation W25502953931 @default.
- W2550295393 hasOpenAccess W2550295393 @default.
- W2550295393 hasPrimaryLocation W25502953931 @default.
- W2550295393 hasRelatedWork W2033111932 @default.
- W2550295393 hasRelatedWork W2158794944 @default.
- W2550295393 hasRelatedWork W216180570 @default.
- W2550295393 hasRelatedWork W2275460402 @default.
- W2550295393 hasRelatedWork W2381279982 @default.
- W2550295393 hasRelatedWork W2390663418 @default.
- W2550295393 hasRelatedWork W2471085516 @default.
- W2550295393 hasRelatedWork W2592295931 @default.
- W2550295393 hasRelatedWork W2594079485 @default.
- W2550295393 hasRelatedWork W2605134932 @default.
- W2550295393 hasRelatedWork W2909232718 @default.
- W2550295393 hasRelatedWork W2921887085 @default.
- W2550295393 hasRelatedWork W3023674305 @default.
- W2550295393 hasRelatedWork W3086299505 @default.
- W2550295393 hasRelatedWork W3150058800 @default.
- W2550295393 hasRelatedWork W3168962282 @default.
- W2550295393 hasRelatedWork W3207026371 @default.
- W2550295393 hasRelatedWork W2185070002 @default.
- W2550295393 hasRelatedWork W3147032153 @default.
- W2550295393 hasRelatedWork W806262044 @default.
- W2550295393 isParatext "false" @default.
- W2550295393 isRetracted "false" @default.
- W2550295393 magId "2550295393" @default.
- W2550295393 workType "article" @default.