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• W2550659105 abstract "Blast disease is caused by the fungus Pyricularia grisea and is a widespread disease in India and other countries affecting different aerial parts of the plant at all stages of its growth starting from seedling to grain formation. Specific DNA technology, such as the use of SCAR (sequence characterized amplified region) markers, can be used to differentiate between resistant and susceptible species of finger millet (Eleusine coracana (L.) Gaertn). SCAR markers were created by sequencing a single RAPD (random amplified polymorphic DNA) band and designing primers to amplify the band of specific size. PCR analysis was done using two F2 bulks (one resistant and another susceptible) and two resistant F3 bulk populations. RAPD primer OPA20 amplified bands of 250 and 750 bp in resistant bulks and absent in susceptible bulks. The fragments were cloned and converted into SCAR markers. Two SCAR primer pairs were designed. Testing was done with 3 resistant cultivars and one susceptible cultivar; single unique bands were correctly amplified for resistant cultivars by SCAR primer pairs. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated." @default.
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• W2550659105 date "2010-01-01" @default.
• W2550659105 modified "2023-09-27" @default.
• W2550659105 title "Development and Evaluation of SCAR Marker for Blast Resistance in Finger Millet (Eleusine coracana (L.) Gaertn)" @default.
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