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• W2550922013 abstract "AbstractIn this study, the synthesis of 3-O-β-D-galactopyranosyl-sn-glycerol (GG) was performed by the reverse hydrolysis of D-galactose and glycerol using β-galactosidase from Kluyveromyces lactis. Four process variables, reaction temperature (30.0–45.0 °C), reaction time (24–48 h), enzyme concentration (150.00–350.00 U/mL), and substrate molar ratio (glycerol:D-galactose, 7.5:12.5 mmol/mmol) were investigated and optimized via response surface methodology (RSM) for optimal GG synthesis. Both quadratic equations and the optimal reaction conditions were established. Results showed that the four variables, i.e., reaction temperature, reaction time, enzyme concentration, and substrate molar ratio had significant (p < 0.0001) effects on the synthesis of GG. The optimal reaction conditions were identified as 350.00 U/mL of β-galactosidase concentration and 8.65:1.00 of substrate molar concentration ratio (glycerol: D-galactose) at 39.8 °C and 48 h of reaction. Under these conditions, the GG concentration was ..." @default.
• W2550922013 created "2016-11-30" @default.
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• W2550922013 date "2016-07-03" @default.
• W2550922013 modified "2023-09-26" @default.
• W2550922013 title "β-galactosidase-catalyzed synthesis of 3-O-β-D-galactopyranosyl-sn-glycerol: Optimization by response surface methodology" @default.
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• W2550922013 doi "https://doi.org/10.1080/10242422.2016.1247815" @default.
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