FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2550994966> ?p ?o ?g. }

• W2550994966 abstract "Background Rheumatoid arthritis (RA) is a chronic autoimmune disease characterised by inflammation of diarthrodial joints. Sensory neurons expressing transient receptor potential (TRP) channels, a wide family of non-selective cation channels, have been shown to play a role in the pathogenesis of RA 1 . Transient receptor potential 5 (TRPC5) is a member of the canonical family of TRPC channels and is abundantly expressed in the central nervous system and periphery 2 , including CD55 + fibroblast-like synoviocytes 3 , but the role of TRPC5 in arthritis is unknown. Objectives To evaluate the for the first time, the contribution of TRPC5 in an in vivo model of arthritis and investigate the role of TRPC5 in the modulation of hyperalgesia and joint inflammation. Methods All experiments were conducted under the guidelines of the United Kingdom Home Office Animals (Scientific Procedures) Act 1986. Age-matched, male 129S1/SvImJ wildtype (WT) and TRPC5 knockout (KO) mice littermates bred from heterozygotic mice provided by Prof. D.E. Clapham, USA 2 . Induction of arthritis using CFA was performed as previously described 1 ; inflammation was allowed to progress to 14 days. Nociceptive tests were carried out weekly. Synovial blood flow was assessed in anaesthetised WT and TRPC5 KO mice using a full-field laser perfusion imager (FLPI) 4 on day 14. Ex vivo analysis included assessment of cellular infiltration in the knee joint, and determination of cytokine concentrations. In separate studies, WT and TRPC5 KO mice received the TRPC4/5 antagonist, ML204 5 (2mg/kg, i.p ., n=6) or vehicle (2% DMSO in saline, n=4) daily following i.art . injection of CFA. Results are presented as mean ± s.e.m. and analysed by 2-way ANOVA and Bonferonni post hoc test. Results WT mice developed primary hyperalgesia with a significant reduction in withdrawal threshold compared to baseline responses (334.7±25.7 vs 452.2±8.2 gram force (gf); p Conclusions Our results reveal a protective ability of TRPC5 in inflammatory joint conditions in vivo . Genetic deletion or pharmacological antagonism of TRPC5 resulted in an exacerbated inflammatory response highlighted by heightened nociceptive responses, increased cellular infiltration and cytokine concentrations in the knee joint. References Fernandes, E.S., et al. 2011. Arthritis Rheum 63, 819–829. Riccio, A., et al. 2009. Cell 137, 761–772. Xu, S.Z., et al. 2008. Nature 451, 69–72 Fernandes, E.S., et al. 2016. Arthritis Res Ther 18(1), 7 Miller, M., et al. 2011. J Biol Chem 286, 33436–46. Acknowledgement Supported by Arthritis Research UK. Disclosure of Interest None declared" @default.
• W2550994966 created "2016-11-30" @default.
• W2550994966 creator A5002367996 @default.
• W2550994966 creator A5012947375 @default.
• W2550994966 creator A5013248208 @default.
• W2550994966 creator A5020239267 @default.
• W2550994966 creator A5035703723 @default.
• W2550994966 creator A5048809177 @default.
• W2550994966 creator A5060503067 @default.
• W2550994966 creator A5089412232 @default.
• W2550994966 date "2016-06-01" @default.
• W2550994966 modified "2023-09-27" @default.
• W2550994966 title "AB0095 Investigating The Role of Transient Receptor Potential Canonical 5 (TRPC5) in Complete Freund's Adjuvant (CFA)-Induced Arthritis" @default.
• W2550994966 doi "https://doi.org/10.1136/annrheumdis-2016-eular.3191" @default.
• W2550994966 hasPublicationYear "2016" @default.
• W2550994966 type Work @default.
• W2550994966 sameAs 2550994966 @default.
• W2550994966 citedByCount "0" @default.
• W2550994966 crossrefType "journal-article" @default.
• W2550994966 hasAuthorship W2550994966A5002367996 @default.
• W2550994966 hasAuthorship W2550994966A5012947375 @default.
• W2550994966 hasAuthorship W2550994966A5013248208 @default.
• W2550994966 hasAuthorship W2550994966A5020239267 @default.
• W2550994966 hasAuthorship W2550994966A5035703723 @default.
• W2550994966 hasAuthorship W2550994966A5048809177 @default.
• W2550994966 hasAuthorship W2550994966A5060503067 @default.
• W2550994966 hasAuthorship W2550994966A5089412232 @default.
• W2550994966 hasConcept C126322002 @default.
• W2550994966 hasConcept C134018914 @default.
• W2550994966 hasConcept C155164980 @default.
• W2550994966 hasConcept C170488646 @default.
• W2550994966 hasConcept C170493617 @default.
• W2550994966 hasConcept C196156954 @default.
• W2550994966 hasConcept C203014093 @default.
• W2550994966 hasConcept C2776914184 @default.
• W2550994966 hasConcept C2777077863 @default.
• W2550994966 hasConcept C2777575956 @default.
• W2550994966 hasConcept C71924100 @default.
• W2550994966 hasConceptScore W2550994966C126322002 @default.
• W2550994966 hasConceptScore W2550994966C134018914 @default.
• W2550994966 hasConceptScore W2550994966C155164980 @default.
• W2550994966 hasConceptScore W2550994966C170488646 @default.
• W2550994966 hasConceptScore W2550994966C170493617 @default.
• W2550994966 hasConceptScore W2550994966C196156954 @default.
• W2550994966 hasConceptScore W2550994966C203014093 @default.
• W2550994966 hasConceptScore W2550994966C2776914184 @default.
• W2550994966 hasConceptScore W2550994966C2777077863 @default.
• W2550994966 hasConceptScore W2550994966C2777575956 @default.
• W2550994966 hasConceptScore W2550994966C71924100 @default.
• W2550994966 hasLocation W25509949661 @default.
• W2550994966 hasOpenAccess W2550994966 @default.
• W2550994966 hasPrimaryLocation W25509949661 @default.
• W2550994966 hasRelatedWork W1545637885 @default.
• W2550994966 hasRelatedWork W1581188730 @default.
• W2550994966 hasRelatedWork W1775400584 @default.
• W2550994966 hasRelatedWork W1965466577 @default.
• W2550994966 hasRelatedWork W1966416804 @default.
• W2550994966 hasRelatedWork W1969258434 @default.
• W2550994966 hasRelatedWork W1975427546 @default.
• W2550994966 hasRelatedWork W2015330944 @default.
• W2550994966 hasRelatedWork W2027137963 @default.
• W2550994966 hasRelatedWork W2064963966 @default.
• W2550994966 hasRelatedWork W2074152542 @default.
• W2550994966 hasRelatedWork W2080817628 @default.
• W2550994966 hasRelatedWork W2089188095 @default.
• W2550994966 hasRelatedWork W2529703202 @default.
• W2550994966 hasRelatedWork W2789363078 @default.
• W2550994966 hasRelatedWork W2964692163 @default.
• W2550994966 hasRelatedWork W2982025227 @default.
• W2550994966 hasRelatedWork W3106145039 @default.
• W2550994966 hasRelatedWork W3143824203 @default.
• W2550994966 hasRelatedWork W3166373503 @default.
• W2550994966 isParatext "false" @default.
• W2550994966 isRetracted "false" @default.
• W2550994966 magId "2550994966" @default.
• W2550994966 workType "article" @default.