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- W2551137348 abstract "The RNA homologous to the yeast transposable element Tyl is one of the more abundant poly(A)+ RNAs in many strains of the yeast Saccharomyces cerevisiae. The 5' and 3' ends of Tyl RNA have been determined from analysis of cDNA. The 5' end is 245 bases into the left 6 sequence measured from the left side of the Tyl element. The 8 sequence is a direct repeat of about 340 base pairs present at each end of the Tyl element. The Tyl transcription includes 93-97 bases of the left 6 sequence and con- tinues through the entire internal portion of the element and through about 295 bases of the right 8 sequence before reaching the 3' end located 38-46 bases from the right side of the right 8 sequence. Because the 8 sequences present at each end of a single Tyl element have identical or very similar DNA sequences, these end points for Tyl RNA raise several questions about the expres- sion of Tyl elements. First, what are the initiation and termi- nation signals, because the Tyl transcript must read through a DNA sequence that is identical to the 3' end at about 50 bases from the 5' end? Second, why is the direction of transcription of the Tyl element opposite to that of genes that are overexpressed after the insertion of a Tyl element? Third, because the Tyl RNA itself has direct repeats of about 45 bases, a structure analogous to retro- virus RNAs, is the Tyl RNA an intermediate in the transposition of Tyl?" @default.
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- W2551137348 date "2016-01-01" @default.
- W2551137348 modified "2023-09-23" @default.
- W2551137348 title "RNA from the yeast transposable e) direct repeats, a structure similar t4" @default.
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