Matches in SemOpenAlex for { <https://semopenalex.org/work/W2551556758> ?p ?o ?g. }
- W2551556758 abstract "Porphyromonas gingivalis is a member of the human oral microbiome abundant in dysbiosis and implicated in the pathogenesis of periodontal (gum) disease. It employs a newly described type-IX secretion system (T9SS) for secretion of virulence factors. Cargo proteins destined for secretion through T9SS carry a recognition signal in the conserved C-terminal domain (CTD), which is removed by sortase PorU during translocation. Here, we identified a novel component of T9SS, PorZ, which is essential for surface exposure of PorU and posttranslational modification of T9SS cargo proteins. These include maturation of enzyme precursors, CTD removal and attachment of anionic lipopolysaccharide for anchorage in the outer membrane. The crystal structure of PorZ revealed two β-propeller domains and a C-terminal β-sandwich domain, which conforms to the canonical CTD architecture. We further documented that PorZ is itself transported to the cell surface via T9SS as a full-length protein with its CTD intact, independently of the presence or activity of PorU. Taken together, our results shed light on the architecture and possible function of a novel component of the T9SS. Knowledge of how T9SS operates will contribute to our understanding of protein secretion as part of host-microbiome interactions by dysbiotic members of the human oral cavity." @default.
- W2551556758 created "2016-11-30" @default.
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- W2551556758 date "2016-11-24" @default.
- W2551556758 modified "2023-09-27" @default.
- W2551556758 title "Structural and functional probing of PorZ, an essential bacterial surface component of the type-IX secretion system of human oral-microbiomic Porphyromonas gingivalis." @default.
- W2551556758 cites W1514298611 @default.
- W2551556758 cites W1556056246 @default.
- W2551556758 cites W1811635141 @default.
- W2551556758 cites W1825352697 @default.
- W2551556758 cites W1836157892 @default.
- W2551556758 cites W1854259035 @default.
- W2551556758 cites W1895718854 @default.
- W2551556758 cites W1943338444 @default.
- W2551556758 cites W1964516944 @default.
- W2551556758 cites W1966262113 @default.
- W2551556758 cites W1976620611 @default.
- W2551556758 cites W1987574124 @default.
- W2551556758 cites W1995033602 @default.
- W2551556758 cites W1997644559 @default.
- W2551556758 cites W1999292622 @default.
- W2551556758 cites W2006481713 @default.
- W2551556758 cites W2010133611 @default.
- W2551556758 cites W2014694459 @default.
- W2551556758 cites W2021545698 @default.
- W2551556758 cites W2026863841 @default.
- W2551556758 cites W2028062032 @default.
- W2551556758 cites W2028911325 @default.
- W2551556758 cites W2030517878 @default.
- W2551556758 cites W2030853726 @default.
- W2551556758 cites W2047606368 @default.
- W2551556758 cites W2047960410 @default.
- W2551556758 cites W2048256811 @default.
- W2551556758 cites W2050608553 @default.
- W2551556758 cites W2053683688 @default.
- W2551556758 cites W2055046750 @default.
- W2551556758 cites W2055593270 @default.
- W2551556758 cites W2056077453 @default.
- W2551556758 cites W2058389174 @default.
- W2551556758 cites W2058443443 @default.
- W2551556758 cites W2059222613 @default.
- W2551556758 cites W2061948662 @default.
- W2551556758 cites W2068974275 @default.
- W2551556758 cites W2073797258 @default.
- W2551556758 cites W2073920564 @default.
- W2551556758 cites W2077601582 @default.
- W2551556758 cites W2079660696 @default.
- W2551556758 cites W2088703269 @default.
- W2551556758 cites W2089226835 @default.
- W2551556758 cites W2095793483 @default.
- W2551556758 cites W2100819581 @default.
- W2551556758 cites W2101220662 @default.
- W2551556758 cites W2103069045 @default.
- W2551556758 cites W2103714813 @default.
- W2551556758 cites W2108921801 @default.
- W2551556758 cites W2108959691 @default.
- W2551556758 cites W2109630790 @default.
- W2551556758 cites W2111897470 @default.
- W2551556758 cites W2113541275 @default.
- W2551556758 cites W2113552067 @default.
- W2551556758 cites W2115371828 @default.
- W2551556758 cites W2122151275 @default.
- W2551556758 cites W2122339645 @default.
- W2551556758 cites W2124026197 @default.
- W2551556758 cites W2124310880 @default.
- W2551556758 cites W2125717174 @default.
- W2551556758 cites W2126677834 @default.
- W2551556758 cites W2127130852 @default.
- W2551556758 cites W2127911529 @default.
- W2551556758 cites W2129663900 @default.
- W2551556758 cites W2132629607 @default.
- W2551556758 cites W2133926420 @default.
- W2551556758 cites W2143609157 @default.
- W2551556758 cites W2144998676 @default.
- W2551556758 cites W2147874841 @default.
- W2551556758 cites W2154197653 @default.
- W2551556758 cites W2154714625 @default.
- W2551556758 cites W2155596249 @default.