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- W2551909473 abstract "Abstract We used soils from a 33-year fertilizer experiment and performed high-throughput pyrosequencing analyses targeting the ammonia monooxygenase alpha subunit ( amoA ) gene (also qPCR and terminal restricted fragment length polymorphism (T-RFLP)) to determine whether and how the composition and structure of ammonia-oxidizing microbes in different particle-size fractions (>2000, 2000–200, 200–63, 63–2 and 2–0.1 μm) have changed in response to chronic inorganic (NPK) and organic (NPKM) fertilizer additions under a rice-wheat rotation. We found fertilization and particle-size fractions had strong effects on abundance and structure of ammonia-oxidizers and potential nitrification activity (PNA) ( P Nitrososphaera and Nitrosospira were the dominant clusters of ammonia-oxidizing archaea (AOA) and bacteria (AOB), respectively. Even though AOA population was hundreds of times greater than AOB, it seemed AOB responded more sensitively to ambient shifts which was convinced by NMDS analyses based on Bray-Curtis distance of TRFs and also of OTUs from 454 pyrosequencing." @default.
- W2551909473 created "2016-11-30" @default.
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- W2551909473 date "2017-02-01" @default.
- W2551909473 modified "2023-10-12" @default.
- W2551909473 title "Variable responses of ammonia oxidizers across soil particle-size fractions affect nitrification in a long-term fertilizer experiment" @default.
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- W2551909473 doi "https://doi.org/10.1016/j.soilbio.2016.11.005" @default.
- W2551909473 hasPublicationYear "2017" @default.
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