FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2552063511> ?p ?o ?g. }

• W2552063511 abstract "Abstract After haploidentical stem cell transplantation, immune recovery is slow due to decaying thymic function and extensive T-cell depletion of the graft which is needed to prevent Graft-versus-Host Disease (GvHD). Consequently, infectious related mortality is about 30–40%. To address this problem, we investigated the efficacy of adoptive immunotherapy after photodynamic purging of alloreactive T cells (ATIR, Kiadis Pharma, Amsterdam, The Netherlands) in preventing GvHD and improving immune reconstitution. The 4,5-dibromorhodamine methyl ester (TH9402) is a photosensitizer structurally similar to rhodamine. When donor T cells are activated with allogeneic cells, they retain TH9402 which becomes highly cytotoxic upon activation with visible light. MLR-activated donor T cells are exposed to a fluorescent-light scanning device to eliminate alloreactive T cells. We designed a protocol which provided 3,260 ± 450 (mean ± SD)-fold allodepletion, full retention of T-regulatory cells, and preservation of pathogen- and leukaemia-specific T-cell responses (against Aspergillus, Candida, Cytomegalovirus (CMV), Adenovirus (ADV), Herpes Simplex Virus (HSV), Varicella Zoster Virus (VZV), Toxoplasma antigens; and against leukaemia cells and leukaemia antigens (WT1 and Ca125)). Optimized protocol conditions are: MLR cell concentration: 3–5 × 10e6/ml; MLR duration: 24 hours; TH9402 concentration: 5 μM; TH9402 incorporation, as measured by median fluorescence index (MFI): 20,000 – 25,000; energy delivery: 0.1 J/cm2. Here we present the preliminary results of a clinical trial. Escalating doses of photodynamically allodeleted donor T cells, i.e., 1.25 × 10e5/Kg, 2.5 × 10e5/Kg, 5 × 10e5/Kg, 1 × 10e6/Kg and 1.25 × 10e6/Kg, were infused into groups of haploidentical transplant recipients. Only 1 patient developed grade III aGvHD at the 1 × 10e6/Kg cell dose and responded to immune suppressive treatment. Immune assessment analyses revealed that infusion of cell doses equal or greater than 5 × 10e5/Kg are associated with significant reconstitution of T-cell counts and appearance of pathogen-specific T-cell responses. One month after infusion, CD4+ and CD8+ T cells were 124 ± 54/cmm and 327 ± 42/cmm (versus 11 ± 4/cmm and 8 ± 4/cmm respectively, in patients receiving T-cell doses below 5 × 10e5/Kg, P = 0.0007). Aspergillus, Candida, CMV, ADV, HSV, VZV, Toxoplasma-specific CD4+ and CD8+ T-cell responses had recovered to frequencies within the normal ranges while they were absent in patients who received T cell doses under 5 × 10e5/Kg (P = 0.0002). In conclusion, this study demonstrates the feasibility, safety and preliminary indications of efficacy of adoptive immunotherapy after photodynamic purging of alloreactive T cells in recipients of haploidentical stem cell transplantation. A larger study will evaluate the impact of these T-cell infusions on transplant related mortality and disease free survival." @default.
• W2552063511 created "2016-11-30" @default.
• W2552063511 creator A5013170851 @default.
• W2552063511 creator A5021123903 @default.
• W2552063511 creator A5021584415 @default.
• W2552063511 creator A5047396232 @default.
• W2552063511 creator A5067405775 @default.
• W2552063511 creator A5070029054 @default.
• W2552063511 creator A5083598934 @default.
• W2552063511 creator A5089614080 @default.
• W2552063511 date "2008-11-16" @default.
• W2552063511 modified "2023-09-30" @default.
• W2552063511 title "Adoptive Immunotherapy after Haploidentical Stem Cell Transplantation with T Cells Allodepleted by Photodynamic Purging" @default.
• W2552063511 doi "https://doi.org/10.1182/blood.v112.11.1157.1157" @default.
• W2552063511 hasPublicationYear "2008" @default.
• W2552063511 type Work @default.
• W2552063511 sameAs 2552063511 @default.
• W2552063511 citedByCount "0" @default.
• W2552063511 crossrefType "journal-article" @default.
• W2552063511 hasAuthorship W2552063511A5013170851 @default.
• W2552063511 hasAuthorship W2552063511A5021123903 @default.
• W2552063511 hasAuthorship W2552063511A5021584415 @default.
• W2552063511 hasAuthorship W2552063511A5047396232 @default.
• W2552063511 hasAuthorship W2552063511A5067405775 @default.
• W2552063511 hasAuthorship W2552063511A5070029054 @default.
• W2552063511 hasAuthorship W2552063511A5083598934 @default.
• W2552063511 hasAuthorship W2552063511A5089614080 @default.
• W2552063511 hasConcept C10205521 @default.
• W2552063511 hasConcept C126322002 @default.
• W2552063511 hasConcept C147483822 @default.
• W2552063511 hasConcept C154317977 @default.
• W2552063511 hasConcept C159047783 @default.
• W2552063511 hasConcept C167672396 @default.
• W2552063511 hasConcept C202751555 @default.
• W2552063511 hasConcept C203014093 @default.
• W2552063511 hasConcept C2776090121 @default.
• W2552063511 hasConcept C2777371436 @default.
• W2552063511 hasConcept C2777701055 @default.
• W2552063511 hasConcept C28328180 @default.
• W2552063511 hasConcept C2911091166 @default.
• W2552063511 hasConcept C54355233 @default.
• W2552063511 hasConcept C55493867 @default.
• W2552063511 hasConcept C71924100 @default.
• W2552063511 hasConcept C86803240 @default.
• W2552063511 hasConcept C8891405 @default.
• W2552063511 hasConcept C90375314 @default.
• W2552063511 hasConceptScore W2552063511C10205521 @default.
• W2552063511 hasConceptScore W2552063511C126322002 @default.
• W2552063511 hasConceptScore W2552063511C147483822 @default.
• W2552063511 hasConceptScore W2552063511C154317977 @default.
• W2552063511 hasConceptScore W2552063511C159047783 @default.
• W2552063511 hasConceptScore W2552063511C167672396 @default.
• W2552063511 hasConceptScore W2552063511C202751555 @default.
• W2552063511 hasConceptScore W2552063511C203014093 @default.
• W2552063511 hasConceptScore W2552063511C2776090121 @default.
• W2552063511 hasConceptScore W2552063511C2777371436 @default.
• W2552063511 hasConceptScore W2552063511C2777701055 @default.
• W2552063511 hasConceptScore W2552063511C28328180 @default.
• W2552063511 hasConceptScore W2552063511C2911091166 @default.
• W2552063511 hasConceptScore W2552063511C54355233 @default.
• W2552063511 hasConceptScore W2552063511C55493867 @default.
• W2552063511 hasConceptScore W2552063511C71924100 @default.
• W2552063511 hasConceptScore W2552063511C86803240 @default.
• W2552063511 hasConceptScore W2552063511C8891405 @default.
• W2552063511 hasConceptScore W2552063511C90375314 @default.
• W2552063511 hasLocation W25520635111 @default.
• W2552063511 hasOpenAccess W2552063511 @default.
• W2552063511 hasPrimaryLocation W25520635111 @default.
• W2552063511 hasRelatedWork W1969994790 @default.
• W2552063511 hasRelatedWork W1979072449 @default.
• W2552063511 hasRelatedWork W2012314771 @default.
• W2552063511 hasRelatedWork W2104294740 @default.
• W2552063511 hasRelatedWork W2526159855 @default.
• W2552063511 hasRelatedWork W2531720369 @default.
• W2552063511 hasRelatedWork W2550272059 @default.
• W2552063511 hasRelatedWork W2561591656 @default.
• W2552063511 hasRelatedWork W2562007823 @default.
• W2552063511 hasRelatedWork W2565347146 @default.
• W2552063511 hasRelatedWork W2565924946 @default.
• W2552063511 hasRelatedWork W2586438624 @default.
• W2552063511 hasRelatedWork W2586834054 @default.
• W2552063511 hasRelatedWork W2587128182 @default.
• W2552063511 hasRelatedWork W2914729211 @default.
• W2552063511 hasRelatedWork W2979667348 @default.
• W2552063511 hasRelatedWork W3028161503 @default.
• W2552063511 hasRelatedWork W3029853382 @default.
• W2552063511 hasRelatedWork W3080834969 @default.
• W2552063511 hasRelatedWork W3087457824 @default.
• W2552063511 isParatext "false" @default.
• W2552063511 isRetracted "false" @default.
• W2552063511 magId "2552063511" @default.
• W2552063511 workType "article" @default.