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- W2552249160 abstract "The binary vector p35SGUSINT mobilized into Agrobacterium strain GV2260 was used for transformation of cotyledonary petiole explants of Brassica juncea (L.) Czern cv. ‘Pusa Jaikisan’ and transient GUS expression was used as the basis for identifying the most appropriate conditions for transformation. Genetic transformation is influenced by a number of factors which affect the efficiency. Some of the crucial factors like explant age, preculture period, and bacterial density and use of non-ethylene agent like silver nitrate were evaluated to optimize a protocol for cotyledonary petiole explants. The efficiency of the method optimized with the GUS construct has also been applied for introducing the osmotin-ferritin construct into B. juncea. The transfer of the foreign GUS gene into B. juncea was demonstrated through GUS assays, PCR and Southern blot analyses. The osmotin-ferritin transformants were confirmed in the T0 and T1 generations for the integration and expression of the osmotin and ferritin transgenes through PCR, Southern and Western blots." @default.
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- W2552249160 date "2007-01-01" @default.
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- W2552249160 title "Optimization of Agrobacterium-Mediated Overexpression of Osmotin-Ferritin Genes in Brassica juncea" @default.
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