FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2552276204> ?p ?o ?g. }

• W2552276204 endingPage "357" @default.
• W2552276204 startingPage "327" @default.
• W2552276204 abstract "We describe methods for studying phospholipase D (PLD) interactions with signaling proteins and modulation of these interactions by the PLD reaction product, phosphatidic acid (PA). PLD is fundamental to the physiological maintenance of cellular/intracellular membranes, protein trafficking, cytoskeletal dynamics, membrane remodeling, cell proliferation, meiotic division and sporulation. PA is an acidic phospholipid involved in the biosynthesis of many other lipids that affects the enzymatic activities of many different signaling proteins via protein–lipid interactions or as a substrate. The involvement of PLD as an effector of protein–protein interactions and downstream signaling via PA-mediated processes has led to the investigation of PA-binding domains in target protein partners. We present here data and protocols detailing the interaction between PLD2–Rac2 interaction and modulation of this interaction by PA. We describe biochemical techniques to measure interactions between PLD, PA, and the small GTPase Rac2, which are associated in the cell. We found two maxima concentrations of PA that contributed to association or dissociation of Rac2 with PLD2, as well as the PLD2 lipase and guanine nucleotide exchange factor (GEF) activities. Fluctuations in the Rac2–PLD2 protein–protein binding interaction facilitate shuttling of Rac2 and/or PLD2 within the cell dependent on local cellular PA concentration. Fluorescence resonance emission transfer stoichiometry for PLD2 and Rac2 binding yielded a 3:1 ratio of Rac2:PLD2. Detection of PA in mammalian cells with a new biosensor showed colocalization in and around the nucleus. We also described methods for quantitation of PA in biological materials by HPLC electrospray ionization tandem mass spectrometry." @default.
• W2552276204 created "2016-11-30" @default.
• W2552276204 creator A5002881392 @default.
• W2552276204 creator A5060684279 @default.
• W2552276204 creator A5081541163 @default.
• W2552276204 date "2017-01-01" @default.
• W2552276204 modified "2023-10-16" @default.
• W2552276204 title "PLD Protein–Protein Interactions With Signaling Molecules and Modulation by PA" @default.
• W2552276204 cites W1487243261 @default.
• W2552276204 cites W1499980909 @default.
• W2552276204 cites W1504375443 @default.
• W2552276204 cites W1544900705 @default.
• W2552276204 cites W1571703626 @default.
• W2552276204 cites W1601541244 @default.
• W2552276204 cites W1970268166 @default.
• W2552276204 cites W1973624256 @default.
• W2552276204 cites W1976302801 @default.
• W2552276204 cites W1978888230 @default.
• W2552276204 cites W1982152426 @default.
• W2552276204 cites W1985567519 @default.
• W2552276204 cites W1986296729 @default.
• W2552276204 cites W1994377649 @default.
• W2552276204 cites W1998553495 @default.
• W2552276204 cites W2000000908 @default.
• W2552276204 cites W2001355405 @default.
• W2552276204 cites W2007700129 @default.
• W2552276204 cites W2011076214 @default.
• W2552276204 cites W2017543679 @default.
• W2552276204 cites W2019429102 @default.
• W2552276204 cites W2022778461 @default.
• W2552276204 cites W2025245932 @default.
• W2552276204 cites W2026653301 @default.
• W2552276204 cites W2027533422 @default.
• W2552276204 cites W2040704781 @default.
• W2552276204 cites W2043294570 @default.
• W2552276204 cites W2044175874 @default.
• W2552276204 cites W2045571319 @default.
• W2552276204 cites W2050621406 @default.
• W2552276204 cites W2059158034 @default.
• W2552276204 cites W2059986736 @default.
• W2552276204 cites W2062940354 @default.
• W2552276204 cites W2066833882 @default.
• W2552276204 cites W2068248498 @default.
• W2552276204 cites W2075280286 @default.
• W2552276204 cites W2076225033 @default.
• W2552276204 cites W2079313436 @default.
• W2552276204 cites W2083923706 @default.
• W2552276204 cites W208451122 @default.
• W2552276204 cites W2085369469 @default.
• W2552276204 cites W2085391208 @default.
• W2552276204 cites W2086827297 @default.
• W2552276204 cites W2092839170 @default.
• W2552276204 cites W2099813504 @default.
• W2552276204 cites W2109240475 @default.
• W2552276204 cites W2115545903 @default.
• W2552276204 cites W2116736523 @default.
• W2552276204 cites W2117226853 @default.
• W2552276204 cites W2120614366 @default.
• W2552276204 cites W2120694601 @default.
• W2552276204 cites W2122354859 @default.
• W2552276204 cites W2124552096 @default.
• W2552276204 cites W2126931581 @default.
• W2552276204 cites W2130946704 @default.
• W2552276204 cites W2142891272 @default.
• W2552276204 cites W2143976331 @default.
• W2552276204 cites W2149187763 @default.
• W2552276204 cites W2151422386 @default.
• W2552276204 cites W2160642940 @default.
• W2552276204 cites W2161648012 @default.
• W2552276204 cites W2166817392 @default.
• W2552276204 cites W2169712438 @default.
• W2552276204 cites W2180680253 @default.
• W2552276204 cites W2284815995 @default.
• W2552276204 cites W2421608912 @default.
• W2552276204 cites W4230075339 @default.
• W2552276204 doi "https://doi.org/10.1016/bs.mie.2016.09.042" @default.
• W2552276204 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28063497" @default.
• W2552276204 hasPublicationYear "2017" @default.
• W2552276204 type Work @default.
• W2552276204 sameAs 2552276204 @default.
• W2552276204 citedByCount "7" @default.
• W2552276204 countsByYear W25522762042018 @default.
• W2552276204 countsByYear W25522762042019 @default.
• W2552276204 countsByYear W25522762042020 @default.
• W2552276204 countsByYear W25522762042021 @default.
• W2552276204 countsByYear W25522762042022 @default.
• W2552276204 countsByYear W25522762042023 @default.
• W2552276204 crossrefType "book-chapter" @default.
• W2552276204 hasAuthorship W2552276204A5002881392 @default.
• W2552276204 hasAuthorship W2552276204A5060684279 @default.
• W2552276204 hasAuthorship W2552276204A5081541163 @default.
• W2552276204 hasConcept C11804247 @default.
• W2552276204 hasConcept C119062480 @default.
• W2552276204 hasConcept C124682485 @default.
• W2552276204 hasConcept C12554922 @default.
• W2552276204 hasConcept C126619667 @default.
• W2552276204 hasConcept C1491633281 @default.
• W2552276204 hasConcept C150555746 @default.

• next