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- W2552382306 abstract "Oxidative processes have critical functions in normal and pathological biology; however, sensing and measuring reactive oxygen species (ROS) is not straightforward and is often accomplished with techniques, such as fluorescent indicators, that are limited by auto-oxidation and nonspecificity. As an alternate approach, we developed gold nanoparticles (AuNPs) that undergo oxidation-induced self-assembly. Citrate-capped AuNPs were synthesized using standard methods. In the presence of a source of ROS and glutathione (GSH, a ubiquitous biological tripeptide), a ligand exchange process occurred on the AuNPs with substitution of GSH for the citrate. Subsequent hydrogen bonding between the GSH groups resulted in self-aggregation of the AuNPs, which was monitored by dynamic light scattering, spectrophotometry, and optoacoustic spectroscopy; the degree of aggregation was proportional to the magnitude of oxidative stress. The AuNP oxidation sensor detects ROS in cell-free and cell culture conditions, and shows promise for measuring oxidative stress in biological systems." @default.
- W2552382306 created "2016-11-30" @default.
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- W2552382306 date "2016-01-01" @default.
- W2552382306 modified "2023-09-23" @default.
- W2552382306 title "Sensing of reactive oxygen species by self-aggregating gold nanoparticle assemblies" @default.
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- W2552382306 doi "https://doi.org/10.1016/b978-0-323-41533-0.00005-2" @default.
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