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• W2552491981 endingPage "457" @default.
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• W2552491981 abstract "The structures of three intermediate states of photosystem II, which is crucial for photosynthesis, have been solved at room temperature, shedding new light on this process. During the conversion of light into energy in plants, photosystem II oxidizes water within a Mn4CaO5 cluster in the oxygen evolving complex (OEC). This process involves five intermediate states that have eluded structural determination until now. Junko Yano and colleagues use a femtosecond X-ray free electron laser (XFEL) to capture three of these states at room temperature. As the structure was solved in the presence of ammonia, a water analogue, the authors are able to conclude that the ammonia-binding Mn site is not a substrate water site. Light-induced oxidation of water by photosystem II (PS II) in plants, algae and cyanobacteria has generated most of the dioxygen in the atmosphere. PS II, a membrane-bound multi-subunit pigment protein complex, couples the one-electron photochemistry at the reaction centre with the four-electron redox chemistry of water oxidation at the Mn4CaO5 cluster in the oxygen-evolving complex (OEC). Under illumination, the OEC cycles through five intermediate S-states (S0 to S4)1, in which S1 is the dark-stable state and S3 is the last semi-stable state before O–O bond formation and O2 evolution2,3. A detailed understanding of the O–O bond formation mechanism remains a challenge, and will require elucidation of both the structures of the OEC in the different S-states and the binding of the two substrate waters to the catalytic site4,5,6. Here we report the use of femtosecond pulses from an X-ray free electron laser (XFEL) to obtain damage-free, room temperature structures of dark-adapted (S1), two-flash illuminated (2F; S3-enriched), and ammonia-bound two-flash illuminated (2F-NH3; S3-enriched) PS II. Although the recent 1.95 Å resolution structure of PS II at cryogenic temperature using an XFEL7 provided a damage-free view of the S1 state, measurements at room temperature are required to study the structural landscape of proteins under functional conditions8,9, and also for in situ advancement of the S-states. To investigate the water-binding site(s), ammonia, a water analogue, has been used as a marker, as it binds to the Mn4CaO5 cluster in the S2 and S3 states10. Since the ammonia-bound OEC is active, the ammonia-binding Mn site is not a substrate water site10,11,12,13. This approach, together with a comparison of the native dark and 2F states, is used to discriminate between proposed O–O bond formation mechanisms." @default.
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• W2552491981 date "2016-11-21" @default.
• W2552491981 modified "2023-10-11" @default.
• W2552491981 title "Structure of photosystem II and substrate binding at room temperature" @default.
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• W2552491981 cites W1966176105 @default.
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• W2552491981 cites W1971536893 @default.
• W2552491981 cites W1976171957 @default.
• W2552491981 cites W1977303228 @default.
• W2552491981 cites W1980180661 @default.
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• W2552491981 cites W1994612614 @default.
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• W2552491981 cites W2000046331 @default.
• W2552491981 cites W2000843938 @default.
• W2552491981 cites W2001744100 @default.
• W2552491981 cites W2001981171 @default.
• W2552491981 cites W2004622851 @default.
• W2552491981 cites W2008072526 @default.
• W2552491981 cites W2009778355 @default.
• W2552491981 cites W2010278274 @default.
• W2552491981 cites W2011999890 @default.
• W2552491981 cites W2012015338 @default.
• W2552491981 cites W2039858738 @default.
• W2552491981 cites W2050982148 @default.
• W2552491981 cites W2052501176 @default.
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