FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2553559368> ?p ?o ?g. }

• W2553559368 abstract "Cell-based therapies such as autologous chondrocyte implantation are promising therapeutic approaches to treat cartilage defects to prevent further cartilage degeneration. To assure consistent quality of cell-based therapeutics, it is important to be able to predict the biological activity of such products. This requires the development of a potency assay, which assesses a characteristic of the cell transplant before implantation that can predict its cartilage regeneration capacity after implantation. In this study, an ex vivo human cartilage repair model was developed as quality assessment tool for potency and applied to co.don’s chondrosphere product, a matrix-associated autologous chondrocyte implant (chondrocyte spheroids) that is in clinical use in Germany. Chondrocyte spheroids were generated from 14 donors, and implanted into a subchondral cartilage defect that was manually generated in human articular cartilage tissue. Implanted spheroids and cartilage tissue were co-cultured ex vivo for 12 weeks to allow regeneration processes to form new tissue within the cartilage defect. Before implantation, spheroid characteristics like glycosaminoglycan production and gene and protein expression of chondrogenic markers were assessed for each donor sample and compared to determine donor-dependent variation. After the co-cultivation, histological analyses showed the formation of repair tissue within the cartilage defect, which varied in amount for the different donors. In the repair tissue, aggrecan protein was expressed and extra-cellular matrix cartilage fibers were present, both indicative for a cartilage hyaline-like character of the repair tissue. The amount of formed repair tissue was used as a read-out for regeneration capacity and was correlated with the spheroid characteristics determined before implantation. A positive correlation was found between high level of aggrecan protein expression in spheroids before implantation and a higher regeneration potential after implantation, reflected by more newly formed repair tissue. This demonstrated that aggrecan protein expression levels in spheroids before implantation can potentially be used as surrogate potency assay for the cartilage cell transplant to predict its regenerative capacity after implantation in human patients." @default.
• W2553559368 created "2016-11-30" @default.
• W2553559368 creator A5002589215 @default.
• W2553559368 creator A5014016854 @default.
• W2553559368 creator A5071428461 @default.
• W2553559368 creator A5083146246 @default.
• W2553559368 creator A5089719995 @default.
• W2553559368 creator A5090228143 @default.
• W2553559368 date "2016-11-15" @default.
• W2553559368 modified "2023-10-16" @default.
• W2553559368 title "An ex vivo human cartilage repair model to evaluate the potency of a cartilage cell transplant" @default.
• W2553559368 cites W1591850913 @default.
• W2553559368 cites W1964895653 @default.
• W2553559368 cites W1972249277 @default.
• W2553559368 cites W1985481249 @default.
• W2553559368 cites W1988592246 @default.
• W2553559368 cites W1994156767 @default.
• W2553559368 cites W2003964325 @default.
• W2553559368 cites W2005140418 @default.
• W2553559368 cites W2005615031 @default.
• W2553559368 cites W2015701996 @default.
• W2553559368 cites W2019849466 @default.
• W2553559368 cites W2032053401 @default.
• W2553559368 cites W2039488563 @default.
• W2553559368 cites W2041401385 @default.
• W2553559368 cites W2041654812 @default.
• W2553559368 cites W204862241 @default.
• W2553559368 cites W2081806906 @default.
• W2553559368 cites W2092613907 @default.
• W2553559368 cites W2101471525 @default.
• W2553559368 cites W2113945572 @default.
• W2553559368 cites W2143979475 @default.
• W2553559368 cites W2170326305 @default.
• W2553559368 cites W2171162651 @default.
• W2553559368 cites W2333321150 @default.
• W2553559368 cites W4255522723 @default.
• W2553559368 cites W6374825 @default.
• W2553559368 doi "https://doi.org/10.1186/s12967-016-1065-8" @default.
• W2553559368 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5111252" @default.
• W2553559368 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27846904" @default.
• W2553559368 hasPublicationYear "2016" @default.
• W2553559368 type Work @default.
• W2553559368 sameAs 2553559368 @default.
• W2553559368 citedByCount "32" @default.
• W2553559368 countsByYear W25535593682018 @default.
• W2553559368 countsByYear W25535593682019 @default.
• W2553559368 countsByYear W25535593682020 @default.
• W2553559368 countsByYear W25535593682021 @default.
• W2553559368 countsByYear W25535593682022 @default.
• W2553559368 countsByYear W25535593682023 @default.
• W2553559368 crossrefType "journal-article" @default.
• W2553559368 hasAuthorship W2553559368A5002589215 @default.
• W2553559368 hasAuthorship W2553559368A5014016854 @default.
• W2553559368 hasAuthorship W2553559368A5071428461 @default.
• W2553559368 hasAuthorship W2553559368A5083146246 @default.
• W2553559368 hasAuthorship W2553559368A5089719995 @default.
• W2553559368 hasAuthorship W2553559368A5090228143 @default.
• W2553559368 hasBestOaLocation W25535593681 @default.
• W2553559368 hasConcept C105702510 @default.
• W2553559368 hasConcept C136229726 @default.
• W2553559368 hasConcept C141071460 @default.
• W2553559368 hasConcept C142724271 @default.
• W2553559368 hasConcept C150903083 @default.
• W2553559368 hasConcept C171056886 @default.
• W2553559368 hasConcept C197534660 @default.
• W2553559368 hasConcept C204787440 @default.
• W2553559368 hasConcept C207001950 @default.
• W2553559368 hasConcept C26291073 @default.
• W2553559368 hasConcept C2776164576 @default.
• W2553559368 hasConcept C2777870964 @default.
• W2553559368 hasConcept C2780024818 @default.
• W2553559368 hasConcept C2780550940 @default.
• W2553559368 hasConcept C2781403057 @default.
• W2553559368 hasConcept C2911091166 @default.
• W2553559368 hasConcept C3020332539 @default.
• W2553559368 hasConcept C35496256 @default.
• W2553559368 hasConcept C49892992 @default.
• W2553559368 hasConcept C71924100 @default.
• W2553559368 hasConcept C86803240 @default.
• W2553559368 hasConcept C95444343 @default.
• W2553559368 hasConceptScore W2553559368C105702510 @default.
• W2553559368 hasConceptScore W2553559368C136229726 @default.
• W2553559368 hasConceptScore W2553559368C141071460 @default.
• W2553559368 hasConceptScore W2553559368C142724271 @default.
• W2553559368 hasConceptScore W2553559368C150903083 @default.
• W2553559368 hasConceptScore W2553559368C171056886 @default.
• W2553559368 hasConceptScore W2553559368C197534660 @default.
• W2553559368 hasConceptScore W2553559368C204787440 @default.
• W2553559368 hasConceptScore W2553559368C207001950 @default.
• W2553559368 hasConceptScore W2553559368C26291073 @default.
• W2553559368 hasConceptScore W2553559368C2776164576 @default.
• W2553559368 hasConceptScore W2553559368C2777870964 @default.
• W2553559368 hasConceptScore W2553559368C2780024818 @default.
• W2553559368 hasConceptScore W2553559368C2780550940 @default.
• W2553559368 hasConceptScore W2553559368C2781403057 @default.
• W2553559368 hasConceptScore W2553559368C2911091166 @default.
• W2553559368 hasConceptScore W2553559368C3020332539 @default.
• W2553559368 hasConceptScore W2553559368C35496256 @default.
• W2553559368 hasConceptScore W2553559368C49892992 @default.
• W2553559368 hasConceptScore W2553559368C71924100 @default.

• next