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• W2553560792 abstract "Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered. Next, three pathways were assembled and the resulting strains synthesized 5-deoxypsicose, 5-deoxysorbose, and 5-deoxyfructose from glucose and glycerol and produce l-fructose, l-tagatose, l-sorbose, and l-psicose with glycerol as the only carbon source. To achieve higher product titer and yield, the recombinant strains were further engineered and fermentation conditions were optimized. Fed-batch culture of engineered strains obtained 38.1 g/L 5-deoxypsicose with a yield of 0.91 ± 0.04 mol product per mol of glycerol and synthesized 20.8 g/L l-fructose, 10.3 g/L l-tagatose, 1.2 g/L l-sorbose, and 0.95 g/L l-psicose." @default.
• W2553560792 created "2016-11-30" @default.
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• W2553560792 date "2016-12-08" @default.
• W2553560792 modified "2023-09-24" @default.
• W2553560792 title "Pathway Construction in <i>Corynebacterium glutamicum</i> and Strain Engineering To Produce Rare Sugars from Glycerol" @default.
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• W2553560792 doi "https://doi.org/10.1021/acs.jafc.6b03423" @default.
• W2553560792 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27998065" @default.
• W2553560792 hasPublicationYear "2016" @default.
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