FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2553566739> ?p ?o ?g. }

• W2553566739 abstract "Purpose This study was carried out to investigate the impact of high concentrations of Clostridium difficile toxin A (TcdA) on the proteome of human cells. It should also be examined whether a catalytically deficient mutant (TcdA NXN ) has an effect on target cells. Experimental design Proteome changes were investigated after treatment of HEp‐2 cells with 20 nM TcdA for 8 h using a triplex SILAC labeling method and shotgun proteomics. Proteins from differently labeled and treated cells were combined for analysis using an HPLC coupled to an Orbitrap mass spectrometer. Results Nearly 4000 proteins were identified in each replicate and 3500 could be quantified by SILAC triplicate analysis. 51 proteins exhibited an altered abundance with 29 up‐regulated and 22 down‐regulated proteins. In contrast, TcdA NXN had no provable impact on the protein profile of HEp‐2 cells. Data analysis of regulated proteins revealed that mainly plasma membrane, cell death, cell proliferation and actin cytoskeleton proteins were affected by TcdA treatment. Conclusions and clinical relevance This proteome analysis showed novel insights of TcdA impact onepithelial cells. Comparison with long‐term treatment studies reveals distinctions in affected cellular processes that will improve the understanding of TcdA functions and might help to find new tools for diagnosis and treatment of CDI." @default.
• W2553566739 created "2016-11-30" @default.
• W2553566739 creator A5001186340 @default.
• W2553566739 creator A5005331384 @default.
• W2553566739 creator A5013560272 @default.
• W2553566739 creator A5023530213 @default.
• W2553566739 creator A5033627937 @default.
• W2553566739 creator A5038812871 @default.
• W2553566739 creator A5066473730 @default.
• W2553566739 date "2016-11-30" @default.
• W2553566739 modified "2023-10-16" @default.
• W2553566739 title "Toxin A of the nosocomial pathogen<i>Clostridium difficile</i>induces primary effects in the proteome of HEp-2 cells" @default.
• W2553566739 cites W1581583867 @default.
• W2553566739 cites W1775749144 @default.
• W2553566739 cites W1824476851 @default.
• W2553566739 cites W1985237848 @default.
• W2553566739 cites W1996690536 @default.
• W2553566739 cites W2002860607 @default.
• W2553566739 cites W2012347803 @default.
• W2553566739 cites W2020462581 @default.
• W2553566739 cites W2036321220 @default.
• W2553566739 cites W2036709958 @default.
• W2553566739 cites W2042802568 @default.
• W2553566739 cites W2053241291 @default.
• W2553566739 cites W2080752012 @default.
• W2553566739 cites W2085700440 @default.
• W2553566739 cites W2102459023 @default.
• W2553566739 cites W2104518766 @default.
• W2553566739 cites W2140946052 @default.
• W2553566739 cites W2157670412 @default.
• W2553566739 cites W2158217645 @default.
• W2553566739 cites W2160456254 @default.
• W2553566739 cites W2162225623 @default.
• W2553566739 cites W2465096192 @default.
• W2553566739 doi "https://doi.org/10.1002/prca.201600031" @default.
• W2553566739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27860399" @default.
• W2553566739 hasPublicationYear "2016" @default.
• W2553566739 type Work @default.
• W2553566739 sameAs 2553566739 @default.
• W2553566739 citedByCount "12" @default.
• W2553566739 countsByYear W25535667392017 @default.
• W2553566739 countsByYear W25535667392018 @default.
• W2553566739 countsByYear W25535667392019 @default.
• W2553566739 countsByYear W25535667392021 @default.
• W2553566739 countsByYear W25535667392022 @default.
• W2553566739 crossrefType "journal-article" @default.
• W2553566739 hasAuthorship W2553566739A5001186340 @default.
• W2553566739 hasAuthorship W2553566739A5005331384 @default.
• W2553566739 hasAuthorship W2553566739A5013560272 @default.
• W2553566739 hasAuthorship W2553566739A5023530213 @default.
• W2553566739 hasAuthorship W2553566739A5033627937 @default.
• W2553566739 hasAuthorship W2553566739A5038812871 @default.
• W2553566739 hasAuthorship W2553566739A5066473730 @default.
• W2553566739 hasConcept C104317684 @default.
• W2553566739 hasConcept C104397665 @default.
• W2553566739 hasConcept C1491633281 @default.
• W2553566739 hasConcept C153911025 @default.
• W2553566739 hasConcept C185592680 @default.
• W2553566739 hasConcept C2994496256 @default.
• W2553566739 hasConcept C34905852 @default.
• W2553566739 hasConcept C46111723 @default.
• W2553566739 hasConcept C501593827 @default.
• W2553566739 hasConcept C55493867 @default.
• W2553566739 hasConcept C68289359 @default.
• W2553566739 hasConcept C86803240 @default.
• W2553566739 hasConcept C89423630 @default.
• W2553566739 hasConcept C95444343 @default.
• W2553566739 hasConcept C97505648 @default.
• W2553566739 hasConceptScore W2553566739C104317684 @default.
• W2553566739 hasConceptScore W2553566739C104397665 @default.
• W2553566739 hasConceptScore W2553566739C1491633281 @default.
• W2553566739 hasConceptScore W2553566739C153911025 @default.
• W2553566739 hasConceptScore W2553566739C185592680 @default.
• W2553566739 hasConceptScore W2553566739C2994496256 @default.
• W2553566739 hasConceptScore W2553566739C34905852 @default.
• W2553566739 hasConceptScore W2553566739C46111723 @default.
• W2553566739 hasConceptScore W2553566739C501593827 @default.
• W2553566739 hasConceptScore W2553566739C55493867 @default.
• W2553566739 hasConceptScore W2553566739C68289359 @default.
• W2553566739 hasConceptScore W2553566739C86803240 @default.
• W2553566739 hasConceptScore W2553566739C89423630 @default.
• W2553566739 hasConceptScore W2553566739C95444343 @default.
• W2553566739 hasConceptScore W2553566739C97505648 @default.
• W2553566739 hasIssue "3-4" @default.
• W2553566739 hasLocation W25535667391 @default.
• W2553566739 hasLocation W25535667392 @default.
• W2553566739 hasOpenAccess W2553566739 @default.
• W2553566739 hasPrimaryLocation W25535667391 @default.
• W2553566739 hasRelatedWork W161698943 @default.
• W2553566739 hasRelatedWork W2001386076 @default.
• W2553566739 hasRelatedWork W2080594197 @default.
• W2553566739 hasRelatedWork W2166040922 @default.
• W2553566739 hasRelatedWork W2586156191 @default.
• W2553566739 hasRelatedWork W28513596 @default.
• W2553566739 hasRelatedWork W2899000927 @default.
• W2553566739 hasRelatedWork W3039408246 @default.
• W2553566739 hasRelatedWork W3204398973 @default.
• W2553566739 hasRelatedWork W4293204701 @default.
• W2553566739 hasVolume "11" @default.
• W2553566739 isParatext "false" @default.

• next