FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2553570983> ?p ?o ?g. }

• W2553570983 abstract "We report the design, construction, and validation of a highly diverse phage-displayed naïve ubiquitin variant (Ubv) library. We first conducted a mutation tolerance scan of 27 residues and confirmed that 24 of these could be substituted by chemically diverse amino acids without compromising the display of Ubvs on phage. Subsequently, we constructed a library containing 6.8×1010 unique members, in which these 24 positions were diversified with a degenerate codon that encodes for 6 aa that are prevalent in protein interaction sites. To ensure the optimal structural stability of the Ubvs, we constructed the library in a two-step process, whereby 12 positions were randomized first, and following the selection for displayed Ubvs, the resulting pool was further diversified at the other 12 positions. The resulting library was validated by conducting binding selections against a panel of 40 diverse protein antigens and was found to be as functional as a highly validated synthetic antibody library, yielding binders against 30 of the antigens. Detailed characterization of an Ubv that bound to the cell-surface receptor human epidermal growth factor receptor 3 revealed tight binding in the single-digit nanomolar range. Moreover, Ubvs that bound to two distinct sites on the intracellular adapter Grb2 could be combined to generate a potent inhibitor that functioned in cells. These results validate ubiquitin as a robust scaffold for the construction of naïve libraries that can be used to generate Ubvs that target signaling networks both outside and inside the cells." @default.
• W2553570983 created "2016-11-30" @default.
• W2553570983 creator A5007632857 @default.
• W2553570983 creator A5044259899 @default.
• W2553570983 creator A5050480664 @default.
• W2553570983 date "2017-01-01" @default.
• W2553570983 modified "2023-10-03" @default.
• W2553570983 title "A Highly Diverse and Functional Naïve Ubiquitin Variant Library for Generation of Intracellular Affinity Reagents" @default.
• W2553570983 cites W1434255190 @default.
• W2553570983 cites W1507805168 @default.
• W2553570983 cites W1592986776 @default.
• W2553570983 cites W1607313147 @default.
• W2553570983 cites W1947732437 @default.
• W2553570983 cites W1965264462 @default.
• W2553570983 cites W1965864591 @default.
• W2553570983 cites W1967882942 @default.
• W2553570983 cites W1968442411 @default.
• W2553570983 cites W1972901972 @default.
• W2553570983 cites W1974389826 @default.
• W2553570983 cites W1977086964 @default.
• W2553570983 cites W1984707191 @default.
• W2553570983 cites W1991858733 @default.
• W2553570983 cites W1993255368 @default.
• W2553570983 cites W1999824494 @default.
• W2553570983 cites W2012536704 @default.
• W2553570983 cites W2013091822 @default.
• W2553570983 cites W2024298562 @default.
• W2553570983 cites W2029881923 @default.
• W2553570983 cites W2034341688 @default.
• W2553570983 cites W2036550297 @default.
• W2553570983 cites W2041542168 @default.
• W2553570983 cites W2045650397 @default.
• W2553570983 cites W2048181659 @default.
• W2553570983 cites W2052093850 @default.
• W2553570983 cites W2054037688 @default.
• W2553570983 cites W2057222401 @default.
• W2553570983 cites W2061657565 @default.
• W2553570983 cites W2069526613 @default.
• W2553570983 cites W2074598493 @default.
• W2553570983 cites W2074857430 @default.
• W2553570983 cites W2075068668 @default.
• W2553570983 cites W2075770210 @default.
• W2553570983 cites W2077812827 @default.
• W2553570983 cites W2083445427 @default.
• W2553570983 cites W2085690948 @default.
• W2553570983 cites W2086889737 @default.
• W2553570983 cites W2089652099 @default.
• W2553570983 cites W2092803887 @default.
• W2553570983 cites W2093416659 @default.
• W2553570983 cites W2093481978 @default.
• W2553570983 cites W2106761321 @default.
• W2553570983 cites W2112391609 @default.
• W2553570983 cites W2112853929 @default.
• W2553570983 cites W2113970177 @default.
• W2553570983 cites W2122225224 @default.
• W2553570983 cites W2122680286 @default.
• W2553570983 cites W2123000304 @default.
• W2553570983 cites W2142722979 @default.
• W2553570983 cites W2148727240 @default.
• W2553570983 cites W2149136324 @default.
• W2553570983 cites W2158347168 @default.
• W2553570983 cites W2159847871 @default.
• W2553570983 cites W2163018106 @default.
• W2553570983 cites W2168887892 @default.
• W2553570983 cites W2197946614 @default.
• W2553570983 cites W2290268596 @default.
• W2553570983 cites W2301123331 @default.
• W2553570983 cites W783708 @default.
• W2553570983 cites W9910234 @default.
• W2553570983 doi "https://doi.org/10.1016/j.jmb.2016.11.016" @default.
• W2553570983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27887869" @default.
• W2553570983 hasPublicationYear "2017" @default.
• W2553570983 type Work @default.
• W2553570983 sameAs 2553570983 @default.
• W2553570983 citedByCount "18" @default.
• W2553570983 countsByYear W25535709832017 @default.
• W2553570983 countsByYear W25535709832018 @default.
• W2553570983 countsByYear W25535709832019 @default.
• W2553570983 countsByYear W25535709832020 @default.
• W2553570983 countsByYear W25535709832021 @default.
• W2553570983 countsByYear W25535709832022 @default.
• W2553570983 countsByYear W25535709832023 @default.
• W2553570983 crossrefType "journal-article" @default.
• W2553570983 hasAuthorship W2553570983A5007632857 @default.
• W2553570983 hasAuthorship W2553570983A5044259899 @default.
• W2553570983 hasAuthorship W2553570983A5050480664 @default.
• W2553570983 hasConcept C104317684 @default.
• W2553570983 hasConcept C111919701 @default.
• W2553570983 hasConcept C143065580 @default.
• W2553570983 hasConcept C147483822 @default.
• W2553570983 hasConcept C153911025 @default.
• W2553570983 hasConcept C159654299 @default.
• W2553570983 hasConcept C167625842 @default.
• W2553570983 hasConcept C177284502 @default.
• W2553570983 hasConcept C185592680 @default.
• W2553570983 hasConcept C186268636 @default.
• W2553570983 hasConcept C25602115 @default.
• W2553570983 hasConcept C2776471321 @default.
• W2553570983 hasConcept C2781377902 @default.
• W2553570983 hasConcept C41008148 @default.

• next