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- W2553635587 abstract "Serratia marcescens has been described as an important cause of ocular infection with high incidence in contact lens-related keratitis. The only published method for molecular typing for S. marcescens is pulsed field gel electrophoresis. The aim of this study was to verify a protocol for PFGE for S. marcescens. Methods Fifty eight strains of multidrug-resistant S. marcescens were selected from a possible outbreak in a Hospital, Gainesville, Florida, USA. The PFGE protocol was performed according to Laboratory Manual of University of Health Network/Mount Sinai Hospital, Department of Microbiology, which included extra washing steps with preheated dH O and SWB with more incubation time at 55°C and using 5µl XbaI restriction enzyme for digestion 2 for each strain. Results using 1µl XbaI enzyme and overnight incubation at 37°C gave unsuccessful results as lanes were smeared. Also using 5µl XbaI enzyme with overnight incubation gave the same unsuccessful results. While using 5µl XbaI enzyme with only 3hours incubation at 37°C gave good results as clear bands could be seen for all strains. Conclusion this protocol has proved its efficacy as the whole genomic DNA of all 58 selected strains of multidrug-resistant Serratia marcescens were seen as clear bands and no lanes were smeared." @default.
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- W2553635587 date "2014-01-01" @default.
- W2553635587 modified "2023-09-27" @default.
- W2553635587 title "Verification of Pulsed Field Gel Electrophoresis (PFGE) Protocol for Molecular Typing of Serratia marcescens" @default.
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- W2553635587 hasPublicationYear "2014" @default.
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