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• W2553712893 abstract "DNA lasers self-amplify optical signals from a DNA analyte as well as thermodynamic differences between sequences, allowing quasi-digital DNA detection. However, these systems have drawbacks, such as relatively large sample consumption and complicated dye labelling. Moreover, although the lasing signal can detect the target DNA, it is superimposed on an unintended fluorescence background, which persists for non-target DNA samples as well. From an optical point of view, it is thus not truly digital detection and requires spectral analysis to identify the target. In this work, we propose and demonstrate an optofluidic laser that has a single layer of DNA molecules as the gain material. A target DNA produces intensive laser emission comparable to existing DNA lasers, while any unnecessary fluorescence background is successfully suppressed. As a result, the target DNA can be detected with a single laser pulse, in a truly digital manner. Since the DNA molecules cover only a single layer on the surface of the laser microcavity, the DNA sample consumption is a few orders of magnitude lower than that of existing DNA lasers. Furthermore, the DNA molecules are stained by simply immersing the microcavity in the intercalating dye solution, and thus the proposed DNA laser is free of any complex dye-labelling process prior to analysis." @default.
• W2553712893 created "2016-11-30" @default.
• W2553712893 creator A5004086841 @default.
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• W2553712893 date "2016-01-01" @default.
• W2553712893 modified "2023-10-14" @default.
• W2553712893 title "Digital DNA detection based on a compact optofluidic laser with ultra-low sample consumption" @default.
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• W2553712893 doi "https://doi.org/10.1039/c6lc01258b" @default.
• W2553712893 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5137248" @default.
• W2553712893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27868127" @default.
• W2553712893 hasPublicationYear "2016" @default.
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